DNA fingerprinting, DNA barcoding, and next generation sequencing technology in plants

Nikolaus J. Sucher, James R. Hennell, Maria C. Carles

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    DNA fingerprinting of plants has become an invaluable tool in forensic, scientific, and industrial laboratories all over the world. PCR has become part of virtually every variation of the plethora of approaches used for DNA fingerprinting today. DNA sequencing is increasingly used either in combination with or as a replacement for traditional DNA fingerprinting techniques. A prime example is the use of short, standardized regions of the genome as taxon barcodes for biological identification of plants. Rapid advances in "next generation sequencing" (NGS) technology are driving down the cost of sequencing and bringing large-scale sequencing projects into the reach of individual investigators. We present an overview of recent publications that demonstrate the use of "NGS" technology for DNA fingerprinting and DNA barcoding applications.
    Original languageEnglish
    Pages (from-to)13-22
    Number of pages10
    JournalMethods in Molecular Biology
    Volume862
    DOIs
    Publication statusPublished - 2012

    Keywords

    • DNA fingerprinting
    • chloroplast DNA
    • nucleotide sequence

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