TY - JOUR
T1 - Dynamics of inflammatory immune response in a mouse model of traumatic spinal cord injury, potential therapeutic targets
AU - Hassanpour Golakani, M.
AU - Mohammad, M.
AU - Li, H.
AU - Saxena, M.
AU - Breit, S.
AU - Ruitenberg, M.
AU - Brown, D.
PY - 2016
Y1 - 2016
N2 - The dynamics of inflammatory responses post spinal cord injury (SCI) are largely unknown. In this study we aimed to characterize the various immune cells in and around the injury site and their relationship with locomotor functional recovery at various time points post SCI. Severe contusive SCI was induced and locomotor function was assessed at 7, 14, 21, and 28 days post injury (dpi). The entire spinal cord was removed, and immune cells were isolated, characterized, and localized using FACS, immunohistology and immunofluorescence. The frequency of immune cell subsets was correlated with behavioral assessment at various time points post injury. The number of infiltrating macrophages and CD11c&B220++ cells had both beneficial and detrimental effects depending on the time post SCI. While macrophages had adverse effects on locomotion recovery in early stages of 7-14 dpi, they significantly contributed to improved recovery at later stages. CD11c&B220++ cells on the other hand were advantageous in early and detrimental in the more chronic stage of SCI. These findings highlight that the dynamics of inflammatory responses post SCI are a double-edge sword with respect to functional recovery, and that they may be therapeutically manipulated.
AB - The dynamics of inflammatory responses post spinal cord injury (SCI) are largely unknown. In this study we aimed to characterize the various immune cells in and around the injury site and their relationship with locomotor functional recovery at various time points post SCI. Severe contusive SCI was induced and locomotor function was assessed at 7, 14, 21, and 28 days post injury (dpi). The entire spinal cord was removed, and immune cells were isolated, characterized, and localized using FACS, immunohistology and immunofluorescence. The frequency of immune cell subsets was correlated with behavioral assessment at various time points post injury. The number of infiltrating macrophages and CD11c&B220++ cells had both beneficial and detrimental effects depending on the time post SCI. While macrophages had adverse effects on locomotion recovery in early stages of 7-14 dpi, they significantly contributed to improved recovery at later stages. CD11c&B220++ cells on the other hand were advantageous in early and detrimental in the more chronic stage of SCI. These findings highlight that the dynamics of inflammatory responses post SCI are a double-edge sword with respect to functional recovery, and that they may be therapeutically manipulated.
UR - https://hdl.handle.net/1959.7/uws:69784
M3 - Article
SN - 0014-2980
VL - 46
SP - 566
EP - 566
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - Suppl. 1
ER -