ECM-chitosan bandage for tissue repair

Antonio Lauto, Leonardo Longo

Research output: Chapter in Book / Conference PaperConference Paperpeer-review

Abstract

Extracellular matrices (ECMs) are currently applied in reconstructive surgery to enhance wound healing and tissue remodelling. Sutures and staples are usually employed to stabilize ECM on tissue although they may damage the matrix structure. In this investigation, a novel biocompatible bandage was developed to implant ECM on tissue without sutures. An adhesive film, based on chitosan, was integrated with small intestine submucosa (SIS) in a single bandage strip. This bandage was bonded to sheep small intestine upon laser irradiation of the chitosan film (P= 0.12W, Fluence = 46±1 J/cm2) to assess tissue adhesion strength. Thermocouples were used to estimate temperatures under SIS during laser irradiation. The bandage successfully bonded to intestine achieving a shear stress of 9.6±1.6 kPa (n=15). During laser irradiation, the temperature increased modestly to 31±2 0C (n=14) beneath the ECM portion of the bandage. The SISchitosan bandage bonded effectively to tissue without sutures and preserved the ECM structure avoiding irreversible thermal denaturation of imbedded bioactive proteins.

Original languageEnglish
Title of host publicationLaser Florence 2009
Subtitle of host publicationA Gallery Through the Laser Medicine World - Selected Papers at the International Laser Medicine Congress
Pages155-160
Number of pages6
DOIs
Publication statusPublished - 2010
EventCongress of the International Academy Laser Medicine and Surgery - Firenze, Italy
Duration: 6 Nov 20097 Nov 2009

Publication series

NameAIP Conference Proceedings
Volume1226
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

ConferenceCongress of the International Academy Laser Medicine and Surgery
Country/TerritoryItaly
CityFirenze
Period6/11/097/11/09

Keywords

  • Biomaterials
  • Laser welding
  • Tissue engineering

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