TY - JOUR
T1 - Effect of vanadium on growth, photosynthesis, reactive oxygen species, antioxidant enzymes, and cell death of rice
AU - Altaf, Muhammad Mohsin
AU - Diao, Xiao-ping
AU - Rehman, Atique ur
AU - Imtiaz, Muhammad
AU - Shakoor, Awais
AU - Altaf, Muhammad Ahsan
AU - Younis, Haseeb
AU - Fu, Pengcheng
AU - Ghani, Muhammad Usman
PY - 2020
Y1 - 2020
N2 - Vanadium (V) as minor concentration is present in various plants and extensively found in soils. The current study was established to assess the response of rice seedlings to different V concentrations and also investigated its toxic effect on growth, photosynthetic assimilation, relative chlorophyll content, SPAD index, ion leakage, enzyme activities, hydrogen peroxide (H2O2), and cell death. The rice seeds were sown in Petri dishes for 8ÃÂ days, and after that, rice seedlings were grown hydroponically in a climate-controlled growth chamber. After 15ÃÂ days of V-treatment, antioxidant enzyme activities, H2O2, protein contents, photosynthetic assimilation, relative chlorophyll content, and cell death were determined by utilizing the Spectrophotometer (Lambda 25 UV/VIS Spectrophotometer), and V accumulation (roots and shoots) was determined by GFAAS (GTA 120). The obtained results showed that all V concentrations significantly decreased the biomass (dry and fresh) and root growth as a result of the reduction in total root length, root tips, root fork, root surface area, and root crossing, and V was more accumulated in roots than shoots. Besides this, enzymatic activities were significantly enhanced under V stress. The findings also confirmed that seedling exposed to V stress had lower tolerance indices, photosynthetic activity, and protein contents while the ion leakage was consistently increased by increasing the V concentrations. The viability of plant cells severely damaged in response to high V stress, and H2O2 induction might be responsible for cell death. Generally, all V doses had a drastic effect on enzyme activities and caused cell death of rice plans. Moreover, the current study demonstrated that V ≥ 35ÃÂ mgÃÂ L−1 caused damaging effects on rice plants.
AB - Vanadium (V) as minor concentration is present in various plants and extensively found in soils. The current study was established to assess the response of rice seedlings to different V concentrations and also investigated its toxic effect on growth, photosynthetic assimilation, relative chlorophyll content, SPAD index, ion leakage, enzyme activities, hydrogen peroxide (H2O2), and cell death. The rice seeds were sown in Petri dishes for 8ÃÂ days, and after that, rice seedlings were grown hydroponically in a climate-controlled growth chamber. After 15ÃÂ days of V-treatment, antioxidant enzyme activities, H2O2, protein contents, photosynthetic assimilation, relative chlorophyll content, and cell death were determined by utilizing the Spectrophotometer (Lambda 25 UV/VIS Spectrophotometer), and V accumulation (roots and shoots) was determined by GFAAS (GTA 120). The obtained results showed that all V concentrations significantly decreased the biomass (dry and fresh) and root growth as a result of the reduction in total root length, root tips, root fork, root surface area, and root crossing, and V was more accumulated in roots than shoots. Besides this, enzymatic activities were significantly enhanced under V stress. The findings also confirmed that seedling exposed to V stress had lower tolerance indices, photosynthetic activity, and protein contents while the ion leakage was consistently increased by increasing the V concentrations. The viability of plant cells severely damaged in response to high V stress, and H2O2 induction might be responsible for cell death. Generally, all V doses had a drastic effect on enzyme activities and caused cell death of rice plans. Moreover, the current study demonstrated that V ≥ 35ÃÂ mgÃÂ L−1 caused damaging effects on rice plants.
UR - https://hdl.handle.net/1959.7/uws:71122
U2 - 10.1007/s42729-020-00330-x
DO - 10.1007/s42729-020-00330-x
M3 - Article
SN - 0718-9508
VL - 20
SP - 2643
EP - 2656
JO - Journal of Soil Science and Plant Nutrition
JF - Journal of Soil Science and Plant Nutrition
IS - 4
ER -