Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides

Nurussaba Khanam; Cheang Khoo; Abdul G. Khan

Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides

Nurussaba Khanam, Cheang Khoo, Abdul G. Khan

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Abstract

In non-rooted shoots regenerated from calluses induced with nine different cyokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM + naphthaleneacetic acid (NAA) 1 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM and BA 10 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM + indole-3-butyric acid (IBA) 0.1 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 ÃƒÆ’Ã†'Ãƒ"šÃ†'ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ¢â‚¬" 10 ÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“4 and 6.5 ÃƒÆ’Ã†'Ãƒ"šÃ†'ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ¢â‚¬" 10 ÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations. No tropane alkaloids were detected in these shoots.

Original language	English
Journal	Australian Journal of Botany
Publication status	Published - 2001

Keywords

Cytokinins
Duboisia myoporoides
calluses
tropanes

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title = "Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides",

abstract = "In non-rooted shoots regenerated from calluses induced with nine different cyokinin{\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}¢{\^a}‚¬{\AA}“auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokinin{\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}¢{\^a}‚¬{\AA}“auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 {\~A}ƒ{\AE}{\textquoteright}{\~A}†'{\~A}ƒ{"}{\v s}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{\AE}{\textquoteright}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{"}{\v s}{\~A}‚{\^A}µM + naphthaleneacetic acid (NAA) 1 {\~A}ƒ{\AE}{\textquoteright}{\~A}†'{\~A}ƒ{"}{\v s}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{\AE}{\textquoteright}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{"}{\v s}{\~A}‚{\^A}µM and BA 10 {\~A}ƒ{\AE}{\textquoteright}{\~A}†'{\~A}ƒ{"}{\v s}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{\AE}{\textquoteright}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{"}{\v s}{\~A}‚{\^A}µM + indole-3-butyric acid (IBA) 0.1 {\~A}ƒ{\AE}{\textquoteright}{\~A}†'{\~A}ƒ{"}{\v s}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{\AE}{\textquoteright}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{"}{\v s}{\~A}‚{\^A}µM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 {\~A}ƒ{\AE}{\textquoteright}{\~A}†'{\~A}ƒ{"}{\v s}{\~A}†'{\~A}ƒ{\AE}{\textquoteright}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{"}{\v s}{\~A}¢{\^a}‚¬{"} 10 {\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}¢{\^a}‚¬{\AA}“4 and 6.5 {\~A}ƒ{\AE}{\textquoteright}{\~A}†'{\~A}ƒ{"}{\v s}{\~A}†'{\~A}ƒ{\AE}{\textquoteright}{\~A}¢{\^a}‚¬{\AA}¡{\~A}ƒ{"}{\v s}{\~A}¢{\^a}‚¬{"} 10 {\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}¢{\^a}‚¬{\AA}“4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokinin{\~A}ƒ{\^A}¢{\~A}¢{"}{\v s}{\^A}¬{\~A}¢{\^a}‚¬{\AA}“auxin combinations. No tropane alkaloids were detected in these shoots.",

keywords = "Cytokinins, Duboisia myoporoides, calluses, tropanes",

author = "Nurussaba Khanam and Cheang Khoo and Khan, {Abdul G.}",

year = "2001",

language = "English",

journal = "Australian Journal of Botany",

issn = "0067-1924",

publisher = "CSIRO",

}

TY - JOUR

T1 - Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides

AU - Khanam, Nurussaba

AU - Khoo, Cheang

AU - Khan, Abdul G.

PY - 2001

Y1 - 2001

N2 - In non-rooted shoots regenerated from calluses induced with nine different cyokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM + naphthaleneacetic acid (NAA) 1 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM and BA 10 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM + indole-3-butyric acid (IBA) 0.1 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 ÃƒÆ’Ã†'Ãƒ"šÃ†'ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ¢â‚¬" 10 ÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“4 and 6.5 ÃƒÆ’Ã†'Ãƒ"šÃ†'ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ¢â‚¬" 10 ÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations. No tropane alkaloids were detected in these shoots.

AB - In non-rooted shoots regenerated from calluses induced with nine different cyokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM + naphthaleneacetic acid (NAA) 1 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM and BA 10 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM + indole-3-butyric acid (IBA) 0.1 ÃƒÆ’Ã†'Ãƒ"šÃ¢â‚¬Å¡ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ‚ÂµM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 ÃƒÆ’Ã†'Ãƒ"šÃ†'ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ¢â‚¬" 10 ÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“4 and 6.5 ÃƒÆ’Ã†'Ãƒ"šÃ†'ÃƒÆ’Ã¢â‚¬Å¡Ãƒ"šÃ¢â‚¬" 10 ÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokininÃƒÂ¢Ã¢"šÂ¬Ã¢â‚¬Å“auxin combinations. No tropane alkaloids were detected in these shoots.

KW - Cytokinins

KW - Duboisia myoporoides

KW - calluses

KW - tropanes

UR - http://handle.uws.edu.au:8081/1959.7/34251

M3 - Article

SN - 0067-1924

JO - Australian Journal of Botany

JF - Australian Journal of Botany

ER -

Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides

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Keywords

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