Effects of cytokinin-auxin combinations on cell arrangement in the basal stems and tropane alkaloid production in cultured non-rooted shoots of Duboisia myoporoides

In non-rooted shoots regenerated from calluses induced with nine different cyokininââ"šÂ¬Ã¢â‚¬Å“auxin combinations, basal stem elongation and the cell arrangement in the basal stem sections depended on the cytokininââ"šÂ¬Ã¢â‚¬Å“auxin combinations used at the callus-induction stage. Elongated basal stems like seedling and large cells in the secondary xylem were found in the non-rooted shoots regenerated from calluses induced with benzyladenine (BA) 10 ÃÆ'Ã"šÃ¢â‚¬Å¡ÃƒÆ’‚Ã"šÃ‚µM + naphthaleneacetic acid (NAA) 1 ÃÆ'Ã"šÃ¢â‚¬Å¡ÃƒÆ’‚Ã"šÃ‚µM and BA 10 ÃÆ'Ã"šÃ¢â‚¬Å¡ÃƒÆ’‚Ã"šÃ‚µM + indole-3-butyric acid (IBA) 0.1 ÃÆ'Ã"šÃ¢â‚¬Å¡ÃƒÆ’‚Ã"šÃ‚µM. Tropane alkaloids were detected in these shoots. The peak hyoscyamine and scopolamine contents were 38.5 ÃÆ'Ã"šÃ†'ÂÃ"šÃ¢â‚¬" 10 ââ"šÂ¬Ã¢â‚¬Å“4 and 6.5 ÃÆ'Ã"šÃ†'ÂÃ"šÃ¢â‚¬" 10 ââ"šÂ¬Ã¢â‚¬Å“4 % dry weight, respectively. Various patterns of vascular regions without large cells in the secondary xylem were found in the basal stem sections of the non-rooted shoots regenerated from calluses induced with the other cytokininââ"šÂ¬Ã¢â‚¬Å“auxin combinations. No tropane alkaloids were detected in these shoots.