Efficient development kit for well-to-chip customization and detection of colorimetric and fluorescence based microfluidic immunoassays

  • R. Gorkin
  • , R. Burger
  • , D. Kurzbuch
  • , G. G. Donohoe
  • , X. Zhang
  • , M. Czugala
  • , F. B. Lopez
  • , S. O'Driscoll
  • , M. Rook
  • , C. McDonagh
  • , D. Diamond
  • , R. O'Kennedy
  • , J. Ducrée

Research output: Chapter in Book / Conference PaperConference Paperpeer-review

3 Citations (Scopus)

Abstract

Microfluidics is advantageous for immunoassay processing (e.g., reducing immunoreagent consumption/assay times) [1], but the fact that most clinically relevant bioassays are developed for well-plate technologies poses significant challenges for integration within μTAS platforms. Assays developed for 96-well plates must be downscaled and adapted for microfluidic settings, involving difficulties in volume and surface chemistry optimization in addition to the inability for microfluidic detection using conventional analytical approaches. To address the need for performing replicate immunoassays in microfluidic environments, we report on the development of translational, slide-based microfluidic cartridges integrated with specialized detection technologies (fluorescence and colorimetric) that enable miniaturization/ analysis of standard assays.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages924-926
Number of pages3
Publication statusPublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Immunoassay
  • Paired emitter detector diode (PEDD)
  • Supercritical angle fluorescence (SAF)

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