Endogenous free radical generation may influence proteolysis in Mitochondria

Roger T. Dean; John K. Pollak

doi:10.1016/0006-291X(85)90296-7

Endogenous free radical generation may influence proteolysis in Mitochondria

Roger T. Dean, John K. Pollak

University of Sydney

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Isolated Mitochondria were allowed to incorporate radioactive amino acids into protein and proteolysis was then measured. In State 4 free radical generation was manipulated by means of respiratory chain blockers and uncouplers. Conditions of enhanced radical flux resulted in accelerated protein breakdown. We suggest that radicals influence proteolysis in cells both directly (by fragmenting proteins) and indirectly (by rendering proteins more susceptible to proteinases).

Original language	English
Pages (from-to)	1082-1089
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	126
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)90296-7
Publication status	Published - 15 Feb 1985
Externally published	Yes

Access to Document

10.1016/0006-291X(85)90296-7

Cite this

@article{e8ec4c84d5134c0a95966db8cab9571d,

title = "Endogenous free radical generation may influence proteolysis in Mitochondria",

abstract = "Isolated Mitochondria were allowed to incorporate radioactive amino acids into protein and proteolysis was then measured. In State 4 free radical generation was manipulated by means of respiratory chain blockers and uncouplers. Conditions of enhanced radical flux resulted in accelerated protein breakdown. We suggest that radicals influence proteolysis in cells both directly (by fragmenting proteins) and indirectly (by rendering proteins more susceptible to proteinases).",

author = "Dean, {Roger T.} and Pollak, {John K.}",

year = "1985",

month = feb,

day = "15",

doi = "10.1016/0006-291X(85)90296-7",

language = "English",

volume = "126",

pages = "1082--1089",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Endogenous free radical generation may influence proteolysis in Mitochondria

AU - Dean, Roger T.

AU - Pollak, John K.

PY - 1985/2/15

Y1 - 1985/2/15

N2 - Isolated Mitochondria were allowed to incorporate radioactive amino acids into protein and proteolysis was then measured. In State 4 free radical generation was manipulated by means of respiratory chain blockers and uncouplers. Conditions of enhanced radical flux resulted in accelerated protein breakdown. We suggest that radicals influence proteolysis in cells both directly (by fragmenting proteins) and indirectly (by rendering proteins more susceptible to proteinases).

AB - Isolated Mitochondria were allowed to incorporate radioactive amino acids into protein and proteolysis was then measured. In State 4 free radical generation was manipulated by means of respiratory chain blockers and uncouplers. Conditions of enhanced radical flux resulted in accelerated protein breakdown. We suggest that radicals influence proteolysis in cells both directly (by fragmenting proteins) and indirectly (by rendering proteins more susceptible to proteinases).

UR - http://www.scopus.com/inward/record.url?scp=0021951263&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(85)90296-7

DO - 10.1016/0006-291X(85)90296-7

M3 - Article

C2 - 3977904

AN - SCOPUS:0021951263

SN - 0006-291X

VL - 126

SP - 1082

EP - 1089

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Endogenous free radical generation may influence proteolysis in Mitochondria

Abstract

Access to Document

Other files and links

Fingerprint

Cite this