Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat

Nicholas G. Paltridge; Paul J. Milham; J. Ivan Ortiz-Monasterio; Govindan Velu; Zarina Yasmin; Lachlan J. Palmer; Georgia E. Guild; James C. R. Stangoulis

doi:10.1007/s11104-012-1423-0

Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat

Nicholas G. Paltridge, Paul J. Milham, J. Ivan Ortiz-Monasterio, Govindan Velu, Zarina Yasmin, Lachlan J. Palmer, Georgia E. Guild, James C. R. Stangoulis

Hawkesbury Institute for the Environment

Research output: Contribution to journal › Article › peer-review

131 Citations (Scopus)

Abstract

Background and aims: Crop biofortification programs require fast, accurate and inexpensive methods of identifying nutrient dense genotypes. This study investigated energy-dispersive X-ray fluorescence spectrometry (EDXRF) for the measurement of zinc (Zn), iron (Fe) and selenium (Se) concentrations in whole grain wheat. Methods: Grain samples were obtained from existing biofortification programs. Reference Zn, Fe and Se concentrations were obtained using inductively coupled plasma optical emission spectrometry (ICP-OES) and/or inductively coupled plasma mass spectrometry (ICP-MS). One set of 25 samples was used to calibrate for Zn (19-60 mg"‰kg-1) and Fe (26-41 mg"‰kg-1), with 25 further samples used to calibrate for Se (2-31 mg"‰kg-1 ). Calibrations were validated using an additional 40-50 wheat samples. Results: EDXRF limits of quantification (LOQ) were estimated as 7, 3 and 2 mg"‰kg-1 for Zn, Fe, and Se, respectively. EDXRF results were highly correlated with ICP-OES or -MS values. Standard errors of EDXRF predictions were Ã‚Â±2.2 mg Zn kg-1, Ã‚Â±2.6 mg Fe kg-1, and Ã‚Â±1.5 mg Se kg-1. Conclusion: EDXRF offers a fast and economical method for the assessment of Zn, Fe and Se concentration in wheat biofortification programs.

Original language	English
Pages (from-to)	261-269
Number of pages	9
Journal	Plant and Soil
Volume	361
Issue number	41306
DOIs	https://doi.org/10.1007/s11104-012-1423-0
Publication status	Published - 2012

Keywords

biofortification
iron
micronutrient
plant
x, ray
zinc

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10.1007/s11104-012-1423-0

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@article{7107e8b8f10b46cf9c76fcf48b20bfb7,

title = "Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat",

abstract = "Background and aims: Crop biofortification programs require fast, accurate and inexpensive methods of identifying nutrient dense genotypes. This study investigated energy-dispersive X-ray fluorescence spectrometry (EDXRF) for the measurement of zinc (Zn), iron (Fe) and selenium (Se) concentrations in whole grain wheat. Methods: Grain samples were obtained from existing biofortification programs. Reference Zn, Fe and Se concentrations were obtained using inductively coupled plasma optical emission spectrometry (ICP-OES) and/or inductively coupled plasma mass spectrometry (ICP-MS). One set of 25 samples was used to calibrate for Zn (19-60 mg{"}‰kg-1) and Fe (26-41 mg{"}‰kg-1), with 25 further samples used to calibrate for Se (2-31 mg{"}‰kg-1 ). Calibrations were validated using an additional 40-50 wheat samples. Results: EDXRF limits of quantification (LOQ) were estimated as 7, 3 and 2 mg{"}‰kg-1 for Zn, Fe, and Se, respectively. EDXRF results were highly correlated with ICP-OES or -MS values. Standard errors of EDXRF predictions were {\~A}‚{\^A}±2.2 mg Zn kg-1, {\~A}‚{\^A}±2.6 mg Fe kg-1, and {\~A}‚{\^A}±1.5 mg Se kg-1. Conclusion: EDXRF offers a fast and economical method for the assessment of Zn, Fe and Se concentration in wheat biofortification programs.",

keywords = "biofortification, iron, micronutrient, plant, x, ray, zinc",

author = "Paltridge, {Nicholas G.} and Milham, {Paul J.} and Ortiz-Monasterio, {J. Ivan} and Govindan Velu and Zarina Yasmin and Palmer, {Lachlan J.} and Guild, {Georgia E.} and Stangoulis, {James C. R.}",

year = "2012",

doi = "10.1007/s11104-012-1423-0",

language = "English",

volume = "361",

pages = "261--269",

journal = "Plant and Soil",

issn = "0032-079X",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "41306",

}

TY - JOUR

T1 - Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat

AU - Paltridge, Nicholas G.

AU - Milham, Paul J.

AU - Ortiz-Monasterio, J. Ivan

AU - Velu, Govindan

AU - Yasmin, Zarina

AU - Palmer, Lachlan J.

AU - Guild, Georgia E.

AU - Stangoulis, James C. R.

PY - 2012

Y1 - 2012

N2 - Background and aims: Crop biofortification programs require fast, accurate and inexpensive methods of identifying nutrient dense genotypes. This study investigated energy-dispersive X-ray fluorescence spectrometry (EDXRF) for the measurement of zinc (Zn), iron (Fe) and selenium (Se) concentrations in whole grain wheat. Methods: Grain samples were obtained from existing biofortification programs. Reference Zn, Fe and Se concentrations were obtained using inductively coupled plasma optical emission spectrometry (ICP-OES) and/or inductively coupled plasma mass spectrometry (ICP-MS). One set of 25 samples was used to calibrate for Zn (19-60 mg"‰kg-1) and Fe (26-41 mg"‰kg-1), with 25 further samples used to calibrate for Se (2-31 mg"‰kg-1 ). Calibrations were validated using an additional 40-50 wheat samples. Results: EDXRF limits of quantification (LOQ) were estimated as 7, 3 and 2 mg"‰kg-1 for Zn, Fe, and Se, respectively. EDXRF results were highly correlated with ICP-OES or -MS values. Standard errors of EDXRF predictions were Ã‚Â±2.2 mg Zn kg-1, Ã‚Â±2.6 mg Fe kg-1, and Ã‚Â±1.5 mg Se kg-1. Conclusion: EDXRF offers a fast and economical method for the assessment of Zn, Fe and Se concentration in wheat biofortification programs.

AB - Background and aims: Crop biofortification programs require fast, accurate and inexpensive methods of identifying nutrient dense genotypes. This study investigated energy-dispersive X-ray fluorescence spectrometry (EDXRF) for the measurement of zinc (Zn), iron (Fe) and selenium (Se) concentrations in whole grain wheat. Methods: Grain samples were obtained from existing biofortification programs. Reference Zn, Fe and Se concentrations were obtained using inductively coupled plasma optical emission spectrometry (ICP-OES) and/or inductively coupled plasma mass spectrometry (ICP-MS). One set of 25 samples was used to calibrate for Zn (19-60 mg"‰kg-1) and Fe (26-41 mg"‰kg-1), with 25 further samples used to calibrate for Se (2-31 mg"‰kg-1 ). Calibrations were validated using an additional 40-50 wheat samples. Results: EDXRF limits of quantification (LOQ) were estimated as 7, 3 and 2 mg"‰kg-1 for Zn, Fe, and Se, respectively. EDXRF results were highly correlated with ICP-OES or -MS values. Standard errors of EDXRF predictions were Ã‚Â±2.2 mg Zn kg-1, Ã‚Â±2.6 mg Fe kg-1, and Ã‚Â±1.5 mg Se kg-1. Conclusion: EDXRF offers a fast and economical method for the assessment of Zn, Fe and Se concentration in wheat biofortification programs.

KW - biofortification

KW - iron

KW - micronutrient

KW - plant

KW - x, ray

KW - zinc

UR - http://handle.uws.edu.au:8081/1959.7/519001

U2 - 10.1007/s11104-012-1423-0

DO - 10.1007/s11104-012-1423-0

M3 - Article

SN - 0032-079X

VL - 361

SP - 261

EP - 269

JO - Plant and Soil

JF - Plant and Soil

IS - 41306

ER -

Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat

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Keywords

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