TY - JOUR
T1 - Ethanol determination using pressure mobilization and free solution capillary electrophoresis by photo-oxidation assisted ultraviolet detection
AU - Oliver, James D.
AU - Gaborieau, Marianne
AU - Castignolles, Patrice
PY - 2014
Y1 - 2014
N2 - Free solution capillary electrophoresis (CE) can separate and quantify carbohydrates using a simple direct UV detection based on a photo-oxidation reaction taking place in the detection window without any labeling. Ethanol interferes with this photo-oxidation reaction. We thus present the first detection and quantification of ethanol using either a simple pressure mobilization set-up or CE. Ethanol can be detected down to 34.9 mg L−1 and quantified in the range 117–1850 mg L−1 through the interference with photo-oxidization of 2 g L−1 sucrose. CE can thus separate and quantify both carbohydrates and ethanol, for example to monitor a lignocellulosic fermentation process. The method is not limited to ethanol and applies to alkyl amines and other alcohols and likely to most molecules possessing the ability to react with free radicals assuming they can be sufficiently separated from each other.
AB - Free solution capillary electrophoresis (CE) can separate and quantify carbohydrates using a simple direct UV detection based on a photo-oxidation reaction taking place in the detection window without any labeling. Ethanol interferes with this photo-oxidation reaction. We thus present the first detection and quantification of ethanol using either a simple pressure mobilization set-up or CE. Ethanol can be detected down to 34.9 mg L−1 and quantified in the range 117–1850 mg L−1 through the interference with photo-oxidization of 2 g L−1 sucrose. CE can thus separate and quantify both carbohydrates and ethanol, for example to monitor a lignocellulosic fermentation process. The method is not limited to ethanol and applies to alkyl amines and other alcohols and likely to most molecules possessing the ability to react with free radicals assuming they can be sufficiently separated from each other.
UR - http://handle.uws.edu.au:8081/1959.7/545171
U2 - 10.1016/j.chroma.2014.04.076
DO - 10.1016/j.chroma.2014.04.076
M3 - Article
SN - 0021-9673
VL - 1348
SP - 150
EP - 157
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -