TY - JOUR
T1 - Ethylene levels are regulated by a plant encoded 1"aminocyclopropane"1"carboxylic acid deaminase
AU - McDonnell, Lisa
AU - Plett, Jonathan M.
AU - Andersson-Gunnerås, Sara
AU - Kozela, Christopher
AU - Dugardeyn, Jasper
AU - Van Der Straeten, Dominique
AU - Glick, Bernard R.
AU - Sundberg, Björn E.
AU - Regan, Sharon
PY - 2009
Y1 - 2009
N2 - Control of the levels of the plant hormone ethylene is crucial in the regulation of many developmental processes and stress responses. Ethylene production can be controlled by altering endogenous levels of 1-aminocyclopropane-1- carboxylic acid (ACC), the immediate precursor to ethylene or by altering its conversion to ethylene. ACC is known to be irreversibly broken down by bacterial or fungal ACC deaminases (ACDs). Sequence analysis revealed two putative ACD genes encoded for in the genome of Arabidopsis thaliana (A. thaliana) and we detected ACD activity in plant extracts. Expression of one of these A. thaliana genes (AtACD1) in bacteria indicated that it had ACD activity. Moreover, transgenic plants harboring antisense constructs of the gene decreased ACD activity to 70% of wild-type (WT) levels, displayed an increased sensitivity to ACC and produced significantly more ethylene. Taken together, these results show that AtACD1 can act as a regulator of ACC levels in A. thaliana.
AB - Control of the levels of the plant hormone ethylene is crucial in the regulation of many developmental processes and stress responses. Ethylene production can be controlled by altering endogenous levels of 1-aminocyclopropane-1- carboxylic acid (ACC), the immediate precursor to ethylene or by altering its conversion to ethylene. ACC is known to be irreversibly broken down by bacterial or fungal ACC deaminases (ACDs). Sequence analysis revealed two putative ACD genes encoded for in the genome of Arabidopsis thaliana (A. thaliana) and we detected ACD activity in plant extracts. Expression of one of these A. thaliana genes (AtACD1) in bacteria indicated that it had ACD activity. Moreover, transgenic plants harboring antisense constructs of the gene decreased ACD activity to 70% of wild-type (WT) levels, displayed an increased sensitivity to ACC and produced significantly more ethylene. Taken together, these results show that AtACD1 can act as a regulator of ACC levels in A. thaliana.
UR - http://handle.uws.edu.au:8081/1959.7/528277
U2 - 10.1111/j.1399-3054.2009.01208.x
DO - 10.1111/j.1399-3054.2009.01208.x
M3 - Article
C2 - 19508369
SN - 0031-9317
VL - 136
SP - 94
EP - 109
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 1
ER -