TY - JOUR
T1 - Evaluation of the two dimensional reversed-phase-reversed-phase separations of low-molecular mass polystyrenes
AU - Gray, Michael J.
AU - Dennis, Gary R.
AU - Slonecker, Patrick J.
AU - Shalliker, Ross A.
PY - 2003
Y1 - 2003
N2 - The resolving power of four reversed-phase-reversed-phase two-dimensional (2D) chromatographic systems was evaluated using information theory (IT) and a geometric approach to factor analysis. The first separation dimension employed a C18 column, while the second separation dimension employed a carbon clad zirconia column. Mobile phases in each dimension were either methanol or acetonitrile. The sample matrix that was employed in this study contained a mixture of 58 components, comprised of stereoisomers and structural isomers of a systematic variation in molecular weight. Each of the components were oligostyrenes, with between two and five configurational repeating units having either n-butyl, sec-butyl or tert-butyl end groups. In the two-dimensional systems employed in this study, between 46 and 49 of the 58 components could be separated, depending on the mobile phase combinations - with apparent resolution that could not possibly be achieved in a single one-dimensional separation. The results from this study indicate that in order to fully evaluate the resolving power of a 2D system multiple methods of analysis that evaluate the separation potential are most appropriate. This becomes increasingly important when the sample contains components that are very closely related and the retention of solutes displays a high degree of solute crowding.
AB - The resolving power of four reversed-phase-reversed-phase two-dimensional (2D) chromatographic systems was evaluated using information theory (IT) and a geometric approach to factor analysis. The first separation dimension employed a C18 column, while the second separation dimension employed a carbon clad zirconia column. Mobile phases in each dimension were either methanol or acetonitrile. The sample matrix that was employed in this study contained a mixture of 58 components, comprised of stereoisomers and structural isomers of a systematic variation in molecular weight. Each of the components were oligostyrenes, with between two and five configurational repeating units having either n-butyl, sec-butyl or tert-butyl end groups. In the two-dimensional systems employed in this study, between 46 and 49 of the 58 components could be separated, depending on the mobile phase combinations - with apparent resolution that could not possibly be achieved in a single one-dimensional separation. The results from this study indicate that in order to fully evaluate the resolving power of a 2D system multiple methods of analysis that evaluate the separation potential are most appropriate. This becomes increasingly important when the sample contains components that are very closely related and the retention of solutes displays a high degree of solute crowding.
KW - Chromatographic analysis
KW - Factor analysis
KW - Information theory
KW - Liquid chromatography
KW - Oligostyrene
KW - Polystyrene
KW - Oligostyrenes
KW - Two-dimensional reversed-phase chromatography
KW - Polystyrenes, low-molecular mass
UR - http://handle.uws.edu.au:8081/1959.7/9854
UR - http://www.scopus.com/inward/record.url?scp=0141792796&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(03)01284-6
DO - 10.1016/S0021-9673(03)01284-6
M3 - Article
SN - 0021-9673
VL - 1015
SP - 89
EP - 98
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -