Evaluation of the two dimensional reversed-phase-reversed-phase separations of low-molecular mass polystyrenes

Michael J. Gray, Gary R. Dennis, Patrick J. Slonecker, Ross A. Shalliker

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    The resolving power of four reversed-phase-reversed-phase two-dimensional (2D) chromatographic systems was evaluated using information theory (IT) and a geometric approach to factor analysis. The first separation dimension employed a C18 column, while the second separation dimension employed a carbon clad zirconia column. Mobile phases in each dimension were either methanol or acetonitrile. The sample matrix that was employed in this study contained a mixture of 58 components, comprised of stereoisomers and structural isomers of a systematic variation in molecular weight. Each of the components were oligostyrenes, with between two and five configurational repeating units having either n-butyl, sec-butyl or tert-butyl end groups. In the two-dimensional systems employed in this study, between 46 and 49 of the 58 components could be separated, depending on the mobile phase combinations - with apparent resolution that could not possibly be achieved in a single one-dimensional separation. The results from this study indicate that in order to fully evaluate the resolving power of a 2D system multiple methods of analysis that evaluate the separation potential are most appropriate. This becomes increasingly important when the sample contains components that are very closely related and the retention of solutes displays a high degree of solute crowding.

    Original languageEnglish
    Pages (from-to)89-98
    Number of pages10
    JournalJournal of Chromatography A
    Volume1015
    Issue number1-2
    DOIs
    Publication statusPublished - 2003

    Keywords

    • Chromatographic analysis
    • Factor analysis
    • Information theory
    • Liquid chromatography
    • Oligostyrene
    • Polystyrene
    • Oligostyrenes
    • Two-dimensional reversed-phase chromatography
    • Polystyrenes, low-molecular mass

    Fingerprint

    Dive into the research topics of 'Evaluation of the two dimensional reversed-phase-reversed-phase separations of low-molecular mass polystyrenes'. Together they form a unique fingerprint.

    Cite this