Evidence against the occurrence of artifacts due to carrier ampholyte-protein binding during isoelectric focusing

Roger T. Dean; Michael Messer

doi:10.1016/S0021-9673(01)82265-2

Evidence against the occurrence of artifacts due to carrier ampholyte-protein binding during isoelectric focusing

Roger T. Dean, Michael Messer

University College London

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The formation of irreversible complexes between carrier ampholyte components and proteins was investigated by gel filtration of mixtures of proteins and radioactively labelled ampholytes. Experiments were performed both with purified proteins (albumin, ferritin, β-glucuronidase) and with a complex mixture of proteins (serum); in no case was binding of ampholytes to proteins detected. Thus the results argue against the occurrence in isoelectric focusing of proteins of artifacts due to such complex formation.

Original language	English
Pages (from-to)	353-358
Number of pages	6
Journal	Journal of Chromatography. A
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(01)82265-2
Publication status	Published - 26 Feb 1975
Externally published	Yes

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10.1016/S0021-9673(01)82265-2

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abstract = "The formation of irreversible complexes between carrier ampholyte components and proteins was investigated by gel filtration of mixtures of proteins and radioactively labelled ampholytes. Experiments were performed both with purified proteins (albumin, ferritin, β-glucuronidase) and with a complex mixture of proteins (serum); in no case was binding of ampholytes to proteins detected. Thus the results argue against the occurrence in isoelectric focusing of proteins of artifacts due to such complex formation.",

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AB - The formation of irreversible complexes between carrier ampholyte components and proteins was investigated by gel filtration of mixtures of proteins and radioactively labelled ampholytes. Experiments were performed both with purified proteins (albumin, ferritin, β-glucuronidase) and with a complex mixture of proteins (serum); in no case was binding of ampholytes to proteins detected. Thus the results argue against the occurrence in isoelectric focusing of proteins of artifacts due to such complex formation.

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Evidence against the occurrence of artifacts due to carrier ampholyte-protein binding during isoelectric focusing

Abstract

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