TY - JOUR
T1 - Experimental induction of AGEs in fetal L132 lung cells changes the level of intracellular cathepsin D
AU - Kasper, Michael
AU - Schinzel, Reinhard
AU - Niwa, Toshimitsi
AU - Münch, Gerald
AU - Witt, Martin
AU - Fehrenbach, Heinz
AU - Wilsch-Bräuninger, Michaela
AU - Pehlke, Kerstin
AU - Hofer, Andreas
AU - Funk, Richard H.W.
PY - 1999/7/22
Y1 - 1999/7/22
N2 - The effect of the carbonyl compound glyoxal on the induction of advanced glycation end products (AGEs) in the fetal epithelial lung cells L132 was investigated using immunohistochemical, immunoelectron microscopic, and biochemical methods. It was found that glyoxal treatment resulted in morphological changes of the cells and in the membranous and cytosolic localization of AGEs such as methyl-glyoxal-derived compounds, N-(carboxymethyllysine) (CML) and imidazolone. The formation of AGEs was accompanied with a change in the intracellular expression of cathepsin D and a loss of enzymatic activity.
AB - The effect of the carbonyl compound glyoxal on the induction of advanced glycation end products (AGEs) in the fetal epithelial lung cells L132 was investigated using immunohistochemical, immunoelectron microscopic, and biochemical methods. It was found that glyoxal treatment resulted in morphological changes of the cells and in the membranous and cytosolic localization of AGEs such as methyl-glyoxal-derived compounds, N-(carboxymethyllysine) (CML) and imidazolone. The formation of AGEs was accompanied with a change in the intracellular expression of cathepsin D and a loss of enzymatic activity.
UR - http://www.scopus.com/inward/record.url?scp=0033595113&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0902
DO - 10.1006/bbrc.1999.0902
M3 - Article
C2 - 10405342
AN - SCOPUS:0033595113
SN - 0006-291X
VL - 261
SP - 175
EP - 182
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -