Expression of the O antigen gene cluster is regulated by RfaH through the JUMPstart sequence

Lei Wang; Slade Jensen; Racquel Hallman; Peter R. Reeves

doi:10.1016/S0378-1097(98)00264-X

Expression of the O antigen gene cluster is regulated by RfaH through the JUMPstart sequence

Lei Wang, Slade Jensen, Racquel Hallman, Peter R. Reeves

University of Sydney

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

O antigen genes are clustered, with a JUMPstart sequence located upstream. JUMPstart is a 39-bp sequence, present upstream of many polysaccharide gene clusters and also upstream of haemolysin and F factor gene clusters. RfaH is known to regulate the expression of E. coli group II capsule, haemolysin, F factor and the outer core of lipopolysaccharide all of which have the JUMPstart sequence, and has been shown to function as a transcriptional antiterminator in some cases. Using lacZ fusions to genes in the O antigen gene cluster of Salmonella enterica serovar Typhimurium, we found that RfaH also regulates the expression of O antigen. We showed that RfaH enhances expression of the 18-kb O antigen gene cluster, with promoter-distal genes affected more dramatically. We also showed that the JUMPstart sequence was required for RfaH function. Copyright (C) 1998 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	201-206
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	165
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(98)00264-X
Publication status	Published - 1 Aug 1998
Externally published	Yes

Keywords

JUMPstart
O antigen expression
Regulation
rfaH

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10.1016/S0378-1097(98)00264-X

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title = "Expression of the O antigen gene cluster is regulated by RfaH through the JUMPstart sequence",

abstract = "O antigen genes are clustered, with a JUMPstart sequence located upstream. JUMPstart is a 39-bp sequence, present upstream of many polysaccharide gene clusters and also upstream of haemolysin and F factor gene clusters. RfaH is known to regulate the expression of E. coli group II capsule, haemolysin, F factor and the outer core of lipopolysaccharide all of which have the JUMPstart sequence, and has been shown to function as a transcriptional antiterminator in some cases. Using lacZ fusions to genes in the O antigen gene cluster of Salmonella enterica serovar Typhimurium, we found that RfaH also regulates the expression of O antigen. We showed that RfaH enhances expression of the 18-kb O antigen gene cluster, with promoter-distal genes affected more dramatically. We also showed that the JUMPstart sequence was required for RfaH function. Copyright (C) 1998 Federation of European Microbiological Societies.",

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AU - Wang, Lei

AU - Jensen, Slade

AU - Hallman, Racquel

AU - Reeves, Peter R.

PY - 1998/8/1

Y1 - 1998/8/1

N2 - O antigen genes are clustered, with a JUMPstart sequence located upstream. JUMPstart is a 39-bp sequence, present upstream of many polysaccharide gene clusters and also upstream of haemolysin and F factor gene clusters. RfaH is known to regulate the expression of E. coli group II capsule, haemolysin, F factor and the outer core of lipopolysaccharide all of which have the JUMPstart sequence, and has been shown to function as a transcriptional antiterminator in some cases. Using lacZ fusions to genes in the O antigen gene cluster of Salmonella enterica serovar Typhimurium, we found that RfaH also regulates the expression of O antigen. We showed that RfaH enhances expression of the 18-kb O antigen gene cluster, with promoter-distal genes affected more dramatically. We also showed that the JUMPstart sequence was required for RfaH function. Copyright (C) 1998 Federation of European Microbiological Societies.

AB - O antigen genes are clustered, with a JUMPstart sequence located upstream. JUMPstart is a 39-bp sequence, present upstream of many polysaccharide gene clusters and also upstream of haemolysin and F factor gene clusters. RfaH is known to regulate the expression of E. coli group II capsule, haemolysin, F factor and the outer core of lipopolysaccharide all of which have the JUMPstart sequence, and has been shown to function as a transcriptional antiterminator in some cases. Using lacZ fusions to genes in the O antigen gene cluster of Salmonella enterica serovar Typhimurium, we found that RfaH also regulates the expression of O antigen. We showed that RfaH enhances expression of the 18-kb O antigen gene cluster, with promoter-distal genes affected more dramatically. We also showed that the JUMPstart sequence was required for RfaH function. Copyright (C) 1998 Federation of European Microbiological Societies.

KW - JUMPstart

KW - O antigen expression

KW - Regulation

KW - rfaH

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ER -

Expression of the O antigen gene cluster is regulated by RfaH through the JUMPstart sequence

Abstract

Keywords

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