Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

Serene El-Kamand; Mar-Dean Du Plessis; Teegan Lawson; Liza Cubeddu; Roland Gamsjaeger

doi:10.1007/978-1-0716-1290-3_14

Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

Serene El-Kamand
, Mar-Dean Du Plessis
, Teegan Lawson
, Liza Cubeddu
, Roland Gamsjaeger

Research output: Chapter in Book / Conference Paper › Chapter

Abstract

Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years, we have used NMR spectroscopy to determine the molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.

Original language	English
Title of host publication	Single Stranded DNA Binding Proteins
Editors	Marcos T. Oliveira
Place of Publication	U.S.
Publisher	Humana Press
Pages	229-240
Number of pages	12
ISBN (Electronic)	9781071612903
ISBN (Print)	9781071612897
DOIs	https://doi.org/10.1007/978-1-0716-1290-3_14
Publication status	Published - 2021

Bibliographical note

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1007/978-1-0716-1290-3_14

Cite this

El-Kamand, S., Du Plessis, M.-D., Lawson, T., Cubeddu, L., & Gamsjaeger, R. (2021). Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A). In M. T. Oliveira (Ed.), Single Stranded DNA Binding Proteins (pp. 229-240). Humana Press. https://doi.org/10.1007/978-1-0716-1290-3_14

@inbook{c78c5885ba2d4f3aaf17e673d2852820,

title = "Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)",

abstract = "Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years, we have used NMR spectroscopy to determine the molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.",

author = "Serene El-Kamand and \{Du Plessis\}, Mar-Dean and Teegan Lawson and Liza Cubeddu and Roland Gamsjaeger",

note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

doi = "10.1007/978-1-0716-1290-3\_14",

language = "English",

isbn = "9781071612897",

pages = "229--240",

editor = "Oliveira, \{Marcos T.\}",

booktitle = "Single Stranded DNA Binding Proteins",

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}

El-Kamand, S, Du Plessis, M-D, Lawson, T, Cubeddu, L & Gamsjaeger, R 2021, Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A). in MT Oliveira (ed.), Single Stranded DNA Binding Proteins. Humana Press, U.S., pp. 229-240. https://doi.org/10.1007/978-1-0716-1290-3_14

Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A). / El-Kamand, Serene; Du Plessis, Mar-Dean; Lawson, Teegan et al.
Single Stranded DNA Binding Proteins. ed. / Marcos T. Oliveira. U.S.: Humana Press, 2021. p. 229-240.

Research output: Chapter in Book / Conference Paper › Chapter

TY - CHAP

T1 - Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

AU - El-Kamand, Serene

AU - Du Plessis, Mar-Dean

AU - Lawson, Teegan

AU - Cubeddu, Liza

AU - Gamsjaeger, Roland

PY - 2021

Y1 - 2021

N2 - Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years, we have used NMR spectroscopy to determine the molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.

AB - Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years, we have used NMR spectroscopy to determine the molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.

UR - https://hdl.handle.net/1959.7/uws:59716

U2 - 10.1007/978-1-0716-1290-3_14

DO - 10.1007/978-1-0716-1290-3_14

M3 - Chapter

C2 - 33847962

SN - 9781071612897

SP - 229

EP - 240

BT - Single Stranded DNA Binding Proteins

A2 - Oliveira, Marcos T.

PB - Humana Press

CY - U.S.

ER -

Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

Abstract

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