Extraction and purification of DNA from wood at various stages of decay for metabarcoding of wood-associated fungi

Jeff R. Powell; Michaela Blyton; Brad Oberle; Gillian L. Powell; Jessica Rigg; Darcy Young; Amy E. Zanne

doi:10.1007/978-1-0716-1040-4_10

Extraction and purification of DNA from wood at various stages of decay for metabarcoding of wood-associated fungi

Jeff R. Powell, Michaela Blyton, Brad Oberle, Gillian L. Powell, Jessica Rigg, Darcy Young, Amy E. Zanne

Western Sydney University

Research output: Chapter in Book / Conference Paper › Chapter

3 Citations (Scopus)

Abstract

Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.

Original language	English
Title of host publication	The Plant Microbiome: Methods and Protocols
Editors	Lilia C. Carvalhais, Paul G. Dennis
Place of Publication	U.S.
Publisher	Humana Press
Pages	113-122
Number of pages	10
ISBN (Electronic)	9781071610404
ISBN (Print)	9781071610398
DOIs	https://doi.org/10.1007/978-1-0716-1040-4_10
Publication status	Published - 2021

Keywords

DNA
biodegradation
fungi
wood

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10.1007/978-1-0716-1040-4_10

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Powell, J. R., Blyton, M., Oberle, B., Powell, G. L., Rigg, J., Young, D., & Zanne, A. E. (2021). Extraction and purification of DNA from wood at various stages of decay for metabarcoding of wood-associated fungi. In L. C. Carvalhais, & P. G. Dennis (Eds.), The Plant Microbiome: Methods and Protocols (pp. 113-122). Humana Press. https://doi.org/10.1007/978-1-0716-1040-4_10

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abstract = "Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.",

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AU - Powell, Jeff R.

AU - Blyton, Michaela

AU - Oberle, Brad

AU - Powell, Gillian L.

AU - Rigg, Jessica

AU - Young, Darcy

AU - Zanne, Amy E.

PY - 2021

Y1 - 2021

N2 - Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.

AB - Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.

KW - DNA

KW - biodegradation

KW - fungi

KW - wood

UR - https://hdl.handle.net/1959.7/uws:57910

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DO - 10.1007/978-1-0716-1040-4_10

M3 - Chapter

SN - 9781071610398

SP - 113

EP - 122

BT - The Plant Microbiome: Methods and Protocols

A2 - Carvalhais, Lilia C.

A2 - Dennis, Paul G.

PB - Humana Press

CY - U.S.

ER -

Extraction and purification of DNA from wood at various stages of decay for metabarcoding of wood-associated fungi

Abstract

Keywords

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