Full integration and automation of immunoassay protocols by rotationally actuated dissolvable film valves

Charles Nwankire, Robert Gorkin, Jonathan Siegrist, Jennifer Gaughran, Di Sien Chan, Jens Ducrée

Research output: Chapter in Book / Conference PaperConference Paperpeer-review

1 Citation (Scopus)

Abstract

Here we describe a fully integrated and automated, multi-step immunoassay protocol utilizing our water-dissolvable films (DFs) actuated, centrifugo-pneumatic valving scheme [1]. This technique establishes a sequence of laboratory unit operations comprising of blood separation, metering, mixing/incubation, and liquid reagent release from on-board reservoirs for washing and detection by mere control of the rotational frequency. During storage at rest and at low rotational frequencies, the sacrificial DF valves provide permanent liquid and vapor barriers. However, beyond a critical burst frequency ranging up to 3500 rotations per minute (rpm), these valves yield by inverting a metastable gas-liquid layer to trigger the wetting and thus dissolution of the DFs.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1665-1667
Number of pages3
Publication statusPublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Burst frequency
  • Centrifugal
  • Dissolvable film
  • Integration
  • Microfluidics
  • Sacrificial valve
  • Valving
  • Vapor barrier

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