Genetic variation of HvCBF genes and their association with salinity tolerance in Tibetan annual wild barley

Dezhi Wu, Long Qiu, Lulu Xu, Lingzhen Ye, Mingxian Chen, Dongfa Sun, Zhonghua Chen, Haitao Zhang, Xiaoli Jin, Fei Dai, Guoping Zhang

    Research output: Contribution to journalArticlepeer-review

    83 Citations (Scopus)

    Abstract

    The evaluation of both the genetic variation and the identification of salinity tolerant accessions of Tibetan annual wild barley (hereafter referred to as Tibetan barley) (Hordeum vulgare L. ssp. Spontaneum and H. vulgare L. ssp. agriocrithum) are essential for discovering and exploiting novel alleles involved in salinity tolerance. In this study, we examined tissue dry biomass and the Na + and K + contents of 188 Tibetan barley accessions in response to salt stress. We investigated the genetic variation of transcription factors HvCBF1, HvCBF3 and HvCBF4 within these accessions, conducting association analysis between these three genes and the respective genotypic salt tolerance. Salt stress significantly reduced shoot and root dry weight by 27.6% to 73.1% in the Tibetan barley lines. HvCBF1, HvCBF3 and HvCBF4 showed diverse sequence variation in amplicon as evident by the identification of single nucleotide polymorphisms (SNPs) and 3, 8 and 13 haplotypes, respectively. Furthermore, the decay of Linkage disequilibrium (LD) of chromosome 5 was 8.9 cM (r 2<0.1). Marker bpb-4891 and haplotype 13 (Ps 610) of the HvCBF4 gene were significantly (P<0.05) and highly significantly (P<0.001) associated with salt tolerance. However, HvCBF1 and HvCBF3 genes were not associated with salinity tolerance. The accessions from haplotype 13 of the HvCBF4 gene showed high salinity tolerance, maintaining significantly lower Na +/K + ratios and higher dry weight. It is thus proposed that these Tibetan barley accessions could be of value for enhancing salinity tolerance in cultivated barley.
    Original languageEnglish
    Pages (from-to)1-10
    Number of pages10
    JournalPLoS One
    Volume6
    Issue number7
    DOIs
    Publication statusPublished - 2011

    Open Access - Access Right Statement

    ©2011 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Keywords

    • barley
    • crops and soils
    • salinity
    • soils

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