Genomic DNA extraction and barcoding of endophytic fungi

Patricia L. Diaz, James R. Hennell, Nikolaus J. Sucher

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Endophytes live inter-and/or intracellularly inside healthy aboveground tissues of plants without causing disease. Endophytic fungi are found in virtually every vascular plant species examined. The origins of this symbiotic relationship between endophytes go back to the emergence of vascular plants. Endophytic fungi receive nutrition and protection from their hosts while the plants benefit from the production of fungal secondary metabolites, which enhance the host plants' resistance to herbivores, pathogens, and various abiotic stresses. Endophytic fungi have attracted increased interest as potential sources of secondary metabolites with agricultural, industrial, and medicinal use. This chapter provides detailed protocols for isolation of genomic DNA from fungal endophytes and its use in polymerase chain reaction-based amplification of the internal transcribed spacer region between the conserved flanking regions of the small and large subunit of ribosomal RNA for barcoding purposes.
    Original languageEnglish
    Pages (from-to)171-179
    Number of pages9
    JournalMethods in Molecular Biology
    Volume862
    DOIs
    Publication statusPublished - 2012

    Keywords

    • endophytes
    • fungal DNA
    • fungal endophytes
    • fungi
    • fusarium oxysporum
    • genetics
    • genome
    • internal transcribed spacer
    • microbiology
    • molecular identification techniques
    • polymerase chain reaction
    • ribosomal
    • symbiosis

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