TY - JOUR
T1 - High-performance liquid chromatography with post-column 2,2'-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples
AU - McDermott, Geoffrey P.
AU - Noonan, Laura K.
AU - Mnatsakanyan, Mariam
AU - Shalliker, R. Andrew
AU - Conlan, Xavier A.
AU - Barnett, Neil W.
AU - Francis, Paul S.
PY - 2010
Y1 - 2010
N2 - An improved post-column 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH response were studied in a univariate approach. Optimum conditions were found to be: 5×10-5M DPPH reagent prepared in a 75% methanol: 25% 40mM citric acid-sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2m×0.25mm i.d. PEEK tubing; detection at 521nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies.
AB - An improved post-column 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH response were studied in a univariate approach. Optimum conditions were found to be: 5×10-5M DPPH reagent prepared in a 75% methanol: 25% 40mM citric acid-sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2m×0.25mm i.d. PEEK tubing; detection at 521nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies.
UR - http://handle.uws.edu.au:8081/1959.7/552876
U2 - 10.1016/j.aca.2010.06.041
DO - 10.1016/j.aca.2010.06.041
M3 - Article
VL - 675
SP - 76
EP - 82
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -