TY - JOUR
T1 - Host and environmental factors influencing 'Candidatus Liberibacter asiaticus' acquisition in Diaphorina citri
AU - Wu, Fengnian
AU - Huang, Jiaquan
AU - Xu, Meirong
AU - Fox, Eduardo G. P.
AU - Beattie, G. Andrew C.
AU - Holford, Paul
AU - Cen, Yijing
AU - Deng, Xiaoling
PY - 2018
Y1 - 2018
N2 - Background: Diaphorina citri is a vector of ‘Candidatus Liberibacter asiaticus’ (CLas) associated with citrus Huanglongbing. In this study, the infection and titers of CLas in the psyllid, were monitored for life cycle stage, sex, host‐plant CLas titer, host‐plant genotype, and ambient temperature. Results: Acquisition efficiency of CLas by D. citri was highest in nymphs reared at 25 °C on a host plant with high CLas titers but was independent of the host genotypes assessed and of vector sex. We further observed that D. citri nymphs acquired CLas more rapidly than adults based on acquisition access periods (AAPs). CLas did not multiply in the alimentary canal, hemolymph, and salivary glands of adults for 18 days after a 3‐day AAP as adult. However, CLas multiplication was detected in hemolymph and salivary gland of adults after the bacterium was acquired by nymphs. Eighty percent of salivary glands of adults contained CLas 18 days after a 3‐day AAP as nymph compared to 10% 18 days after a 3‐day AAP as adults. Conclusion:Different factors tested herein influenced CLas acquisition efficiency of D. citri, CLas multiplication and spread inside the psyllid. These observations serve to better understand mechanisms of CLas infection in D. citri.
AB - Background: Diaphorina citri is a vector of ‘Candidatus Liberibacter asiaticus’ (CLas) associated with citrus Huanglongbing. In this study, the infection and titers of CLas in the psyllid, were monitored for life cycle stage, sex, host‐plant CLas titer, host‐plant genotype, and ambient temperature. Results: Acquisition efficiency of CLas by D. citri was highest in nymphs reared at 25 °C on a host plant with high CLas titers but was independent of the host genotypes assessed and of vector sex. We further observed that D. citri nymphs acquired CLas more rapidly than adults based on acquisition access periods (AAPs). CLas did not multiply in the alimentary canal, hemolymph, and salivary glands of adults for 18 days after a 3‐day AAP as adult. However, CLas multiplication was detected in hemolymph and salivary gland of adults after the bacterium was acquired by nymphs. Eighty percent of salivary glands of adults contained CLas 18 days after a 3‐day AAP as nymph compared to 10% 18 days after a 3‐day AAP as adults. Conclusion:Different factors tested herein influenced CLas acquisition efficiency of D. citri, CLas multiplication and spread inside the psyllid. These observations serve to better understand mechanisms of CLas infection in D. citri.
KW - citrus
KW - greening disease
KW - jumping plant, lice
KW - plant, pathogen relationships
UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:48723
U2 - 10.1002/ps.5060
DO - 10.1002/ps.5060
M3 - Article
VL - 74
SP - 2738
EP - 2746
JO - Pest Management Science
JF - Pest Management Science
IS - 12
ER -