hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress

Nicolas Paquet; Mark N. Adams; Nicholas W. Ashton; Christine Touma; Roland Gamsjaeger; Liza Cubeddu; Vincent Leong; Sam Beard; Emma Bolderson; Catherine H. Botting; Kenneth J. O’Byrne; Derek J. Richard

hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress

Nicolas Paquet, Mark N. Adams, Nicholas W. Ashton, Christine Touma, Roland Gamsjaeger, Liza Cubeddu, Vincent Leong, Sam Beard, Emma Bolderson, Catherine H. Botting, Kenneth J. O’Byrne, Derek J. Richard

Western Sydney University

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.

Original language	English
Article number	27446
Number of pages	13
Journal	Scientific Reports
Volume	6
Publication status	Published - 8 Jun 2016

Keywords

DNA damage
diseases
genomes
nucleic acids
oxidative stress

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress",

abstract = "The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.",

keywords = "DNA damage, diseases, genomes, nucleic acids, oxidative stress",

author = "Nicolas Paquet and Adams, {Mark N.} and Ashton, {Nicholas W.} and Christine Touma and Roland Gamsjaeger and Liza Cubeddu and Vincent Leong and Sam Beard and Emma Bolderson and Botting, {Catherine H.} and O{\textquoteright}Byrne, {Kenneth J.} and Richard, {Derek J.}",

year = "2016",

month = jun,

day = "8",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

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TY - JOUR

T1 - hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress

AU - Paquet, Nicolas

AU - Adams, Mark N.

AU - Ashton, Nicholas W.

AU - Touma, Christine

AU - Gamsjaeger, Roland

AU - Cubeddu, Liza

AU - Leong, Vincent

AU - Beard, Sam

AU - Bolderson, Emma

AU - Botting, Catherine H.

AU - O’Byrne, Kenneth J.

AU - Richard, Derek J.

PY - 2016/6/8

Y1 - 2016/6/8

N2 - The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.

AB - The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.

KW - DNA damage

KW - diseases

KW - genomes

KW - nucleic acids

KW - oxidative stress

UR - http://handle.uws.edu.au:8081/1959.7/uws:36058

M3 - Article

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 27446

ER -

hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress

Abstract

Keywords

UN SDGs

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