Identification and determination of the major constituents in traditional Chinese medicinal plant Polygonum multiflorum Thunb by HPLC coupled with PAD and ESI/MS

Tao Yi, Kelvin S. Y. Leung, Guanghua Lu, Hao Zhang, Kelvin Chan

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    82 Citations (Scopus)

    Abstract

    An HPLC–PAD–MS method has been developed for the qualitative and quantitative analysis of the major chemical constituents in Polygonum multiflorum Thunb. Chromatographic separation was conducted on an Alltima C18 column using water:acetonitrile:acetic acid as the mobile phase. Altogether nine compounds of various classes, including stilbene glucosides, anthraquinone glucosides and anthraquinone derivates, were identified by online ESI/MS. Their identities were ascertained by comparison with data derived from the literature and/or standard compounds. Five components were quantified by HPLC–PAD and the method was fully validated. All the linear regressions were acquired with R2 0.99 and the quantification limits (with a signal-to-noise ratio of 3) ranged from 0.63 and 1.57 ng. Repeatability was evaluated by intra- and inter-day assays and RSD value was within 2.47%. Recovery studies for the quantified compounds were found to be within the range 96.32–102.53% with RSD less than 2.35%. The overall procedure is rapid and reproducible and is considered suitable for qualitative and quantitative analysis of a large number of samples.
    Original languageEnglish
    Pages (from-to)181-187
    Number of pages7
    JournalPhytochemical Analysis
    Volume18
    Issue number3
    DOIs
    Publication statusPublished - 2007

    Keywords

    • HPLC
    • Polygonaceae

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