Identification of Chromera velia by fluorescence in situ hybridization

Victoria Morin-Adeline, Christie Foster, Jan Slapeta

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Chromera velia is evolutionarily the closest free-living and photosynthetic organism to the medically important obligatory parasitic apicomplexans that cause diseases including malaria and toxoplasmosis. In this study, a novel oligonucleotide probe targeting C. velia's small subunit ribosomal RNA was designed. To enable usage of this probe as a detection tool, a fluorescence in situ hybridization (FISH) protocol was optimized. The results obtained showed that when used in combination, the C. velia CV1 probe and optimized FISH protocol enabled efficient detection of C. velia in culture. This new technique will allow a better understanding of the ecological role of C. velia within the coral microhabitat.
Original languageEnglish
Pages (from-to)144-149
Number of pages6
JournalFEMS Microbiology Letters
Volume328
Issue number2
DOIs
Publication statusPublished - 2012

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