TY - JOUR
T1 - Identification of Chromera velia by fluorescence in situ hybridization
AU - Morin-Adeline, Victoria
AU - Foster, Christie
AU - Slapeta, Jan
PY - 2012
Y1 - 2012
N2 - Chromera velia is evolutionarily the closest free-living and photosynthetic organism to the medically important obligatory parasitic apicomplexans that cause diseases including malaria and toxoplasmosis. In this study, a novel oligonucleotide probe targeting C. velia's small subunit ribosomal RNA was designed. To enable usage of this probe as a detection tool, a fluorescence in situ hybridization (FISH) protocol was optimized. The results obtained showed that when used in combination, the C. velia CV1 probe and optimized FISH protocol enabled efficient detection of C. velia in culture. This new technique will allow a better understanding of the ecological role of C. velia within the coral microhabitat.
AB - Chromera velia is evolutionarily the closest free-living and photosynthetic organism to the medically important obligatory parasitic apicomplexans that cause diseases including malaria and toxoplasmosis. In this study, a novel oligonucleotide probe targeting C. velia's small subunit ribosomal RNA was designed. To enable usage of this probe as a detection tool, a fluorescence in situ hybridization (FISH) protocol was optimized. The results obtained showed that when used in combination, the C. velia CV1 probe and optimized FISH protocol enabled efficient detection of C. velia in culture. This new technique will allow a better understanding of the ecological role of C. velia within the coral microhabitat.
UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:42017
U2 - 10.1111/j.1574-6968.2011.02489.x
DO - 10.1111/j.1574-6968.2011.02489.x
M3 - Article
SN - 0378-1097
VL - 328
SP - 144
EP - 149
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -