Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum

David M. Chen; Brigitte A. Bastias; Andrew F. S. Taylor; John W. G. Cairney

doi:10.1046/j.1469-8137.2003.00687.x

Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum

David M. Chen, Brigitte A. Bastias, Andrew F. S. Taylor, John W. G. Cairney

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Abstract

Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by polymerase chain reaction (PCR) using primers for white rot fungal laccase genes, and expression of the genes was examined by reverse transcriptase polymerase chain reaction (RT-PCR) for Piloderma byssinum in axenic culture under different nutrient conditions. Laccase-like genes were present in Rhizopogon roseolus along with several Russulales and Atheliaceae taxa, and showed strong nucleotide sequence similarity to laccase genes in white rot fungi. Multiple laccase-like genes were only identified in Piloderma spp. Laccase-like genes were expressed in Piloderma spp., with transcript levels some six times higher under high nitrogen conditions in P. byssinum than when nitrogen availability was lower. The potential roles of laccases in nutrient mobilization and/or differentiation of multihyphal ECM fungal structures are discussed.

Original language	English
Number of pages	8
Journal	New Phytologist
DOIs	https://doi.org/10.1046/j.1469-8137.2003.00687.x
Publication status	Published - 2003

Keywords

Polyphenol oxidase
ectomycorrhizal fungi
gene expression
laccase
microbiological synthesis

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10.1046/j.1469-8137.2003.00687.x

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@article{163bcb33b414445a8b705087755318a6,

title = "Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum",

abstract = "Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by polymerase chain reaction (PCR) using primers for white rot fungal laccase genes, and expression of the genes was examined by reverse transcriptase polymerase chain reaction (RT-PCR) for Piloderma byssinum in axenic culture under different nutrient conditions. Laccase-like genes were present in Rhizopogon roseolus along with several Russulales and Atheliaceae taxa, and showed strong nucleotide sequence similarity to laccase genes in white rot fungi. Multiple laccase-like genes were only identified in Piloderma spp. Laccase-like genes were expressed in Piloderma spp., with transcript levels some six times higher under high nitrogen conditions in P. byssinum than when nitrogen availability was lower. The potential roles of laccases in nutrient mobilization and/or differentiation of multihyphal ECM fungal structures are discussed.",

keywords = "Polyphenol oxidase, ectomycorrhizal fungi, gene expression, laccase, microbiological synthesis",

author = "Chen, {David M.} and Bastias, {Brigitte A.} and Taylor, {Andrew F. S.} and Cairney, {John W. G.}",

year = "2003",

doi = "10.1046/j.1469-8137.2003.00687.x",

language = "English",

journal = "New Phytologist",

issn = "0028-646X",

publisher = "Wiley-Blackwell Publishing",

}

TY - JOUR

T1 - Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum

AU - Chen, David M.

AU - Bastias, Brigitte A.

AU - Taylor, Andrew F. S.

AU - Cairney, John W. G.

PY - 2003

Y1 - 2003

N2 - Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by polymerase chain reaction (PCR) using primers for white rot fungal laccase genes, and expression of the genes was examined by reverse transcriptase polymerase chain reaction (RT-PCR) for Piloderma byssinum in axenic culture under different nutrient conditions. Laccase-like genes were present in Rhizopogon roseolus along with several Russulales and Atheliaceae taxa, and showed strong nucleotide sequence similarity to laccase genes in white rot fungi. Multiple laccase-like genes were only identified in Piloderma spp. Laccase-like genes were expressed in Piloderma spp., with transcript levels some six times higher under high nitrogen conditions in P. byssinum than when nitrogen availability was lower. The potential roles of laccases in nutrient mobilization and/or differentiation of multihyphal ECM fungal structures are discussed.

AB - Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by polymerase chain reaction (PCR) using primers for white rot fungal laccase genes, and expression of the genes was examined by reverse transcriptase polymerase chain reaction (RT-PCR) for Piloderma byssinum in axenic culture under different nutrient conditions. Laccase-like genes were present in Rhizopogon roseolus along with several Russulales and Atheliaceae taxa, and showed strong nucleotide sequence similarity to laccase genes in white rot fungi. Multiple laccase-like genes were only identified in Piloderma spp. Laccase-like genes were expressed in Piloderma spp., with transcript levels some six times higher under high nitrogen conditions in P. byssinum than when nitrogen availability was lower. The potential roles of laccases in nutrient mobilization and/or differentiation of multihyphal ECM fungal structures are discussed.

KW - Polyphenol oxidase

KW - ectomycorrhizal fungi

KW - gene expression

KW - laccase

KW - microbiological synthesis

UR - http://handle.uws.edu.au:8081/1959.7/9993

U2 - 10.1046/j.1469-8137.2003.00687.x

DO - 10.1046/j.1469-8137.2003.00687.x

M3 - Article

SN - 0028-646X

JO - New Phytologist

JF - New Phytologist

ER -

Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum

Abstract

Keywords

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