Immunohistochemical expression of the nicotinic acetylcholine receptor (nAChR) subunits in the human placenta, and effects of cigarette smoking and preeclampsia

R. Machaalani, E. Ghazavi, T. Hinton, A. Makris, A. Hennessy

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Introduction: Cigarette smoking (CS) and preeclampsia (PE), regulate the expression of nicotinic acetylcholine receptor (nAChR) subunits in the placenta, yet no data exist at the histological level. Methods: Using immunohistochemistry of formalin fixed and paraffin embedded placental tissue, this study quantified the expression of nine nAChR subunits (α2, α3, α4, α5, α7, α9, β1, β2, δ) and compared the expression amongst four groups of non-smoker non-PE (controls, n = 8), smokers (n = 8), PE (n = 8), and those who were smokers with PE (smoke + PE, n = 4). Quantification was of the percentage of villi with positive cells stained (% villi with +ve), percentage of positive stained cells per villous (% +ve cells/villous), percentage of positive cells in the decidua (%+ve Decidua), and intensity of staining in the outer villous trophoblast layer. Results: Changes were restricted to the villi (as opposed to the decidua), and were specific to the α9 (smoke + PE), β1 (smokers), and β2 (PE) subunits when compared to controls. CS seemed to have a protective effect for the β2 subunit and an additive effect for the α9 and β1 subunits within the villous core/stroma cells and not the trophoblast layer. Discussion: These findings support that both CS and PE affect nAChRs in the placenta, but that this is restricted to the villi.
Original languageEnglish
Pages (from-to)16-23
Number of pages8
JournalPlacenta
Volume71
DOIs
Publication statusPublished - 2018

Keywords

  • infants
  • nicotine
  • nicotinic receptors
  • placenta
  • preeclampsia
  • tobacco smoke

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