Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor

Leon Caly; Nitin K. Saksena; Sabine C. Piller; David A. Jans

doi:10.1186/1742-4690-5-67

Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor

Leon Caly, Nitin K. Saksena, Sabine C. Piller, David A. Jans

School of Science

Research output: Contribution to journal › Article

30 Citations (Scopus)

Abstract

We previously reported an epidemiologically linked HIV-1 infected patient cohort in which a long term non-progressor (LTNP) infected two recipients who then exhibited normal disease progression. Expression of patient-derived vpr sequences from each of the three cohort members in mammalian cells tagged with GFP revealed a significant reduction in Vpr nuclear import and virion incorporation uniquely from the LTNP, whereas Vpr from the two progressing recipients displayed normal localisation and virion incorporation, implying a link between efficient Vpr nuclear import and HIV disease progression. Importantly, an F72L point mutation in the LTNP was identified for the first time as being uniquely responsible for decreased Vpr nuclear import.

Original language	English
Article number	67
Number of pages	8
Journal	Retrovirology
Volume	5
DOIs	https://doi.org/10.1186/1742-4690-5-67
Publication status	Published - 2008

Open Access - Access Right Statement

Ã‚Â© 2008 Caly et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

HIV (viruses)
Viral Protein R
epidemiology
infection
mutation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/1742-4690-5-67

Cite this

@article{4b4e747724eb4c459d6c159b6b52fc20,

title = "Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor",

abstract = "We previously reported an epidemiologically linked HIV-1 infected patient cohort in which a long term non-progressor (LTNP) infected two recipients who then exhibited normal disease progression. Expression of patient-derived vpr sequences from each of the three cohort members in mammalian cells tagged with GFP revealed a significant reduction in Vpr nuclear import and virion incorporation uniquely from the LTNP, whereas Vpr from the two progressing recipients displayed normal localisation and virion incorporation, implying a link between efficient Vpr nuclear import and HIV disease progression. Importantly, an F72L point mutation in the LTNP was identified for the first time as being uniquely responsible for decreased Vpr nuclear import.",

keywords = "HIV (viruses), Viral Protein R, epidemiology, infection, mutation",

author = "Leon Caly and Saksena, {Nitin K.} and Piller, {Sabine C.} and Jans, {David A.}",

year = "2008",

doi = "10.1186/1742-4690-5-67",

language = "English",

volume = "5",

journal = "Retrovirology",

issn = "1742-4690",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor

AU - Caly, Leon

AU - Saksena, Nitin K.

AU - Piller, Sabine C.

AU - Jans, David A.

PY - 2008

Y1 - 2008

N2 - We previously reported an epidemiologically linked HIV-1 infected patient cohort in which a long term non-progressor (LTNP) infected two recipients who then exhibited normal disease progression. Expression of patient-derived vpr sequences from each of the three cohort members in mammalian cells tagged with GFP revealed a significant reduction in Vpr nuclear import and virion incorporation uniquely from the LTNP, whereas Vpr from the two progressing recipients displayed normal localisation and virion incorporation, implying a link between efficient Vpr nuclear import and HIV disease progression. Importantly, an F72L point mutation in the LTNP was identified for the first time as being uniquely responsible for decreased Vpr nuclear import.

AB - We previously reported an epidemiologically linked HIV-1 infected patient cohort in which a long term non-progressor (LTNP) infected two recipients who then exhibited normal disease progression. Expression of patient-derived vpr sequences from each of the three cohort members in mammalian cells tagged with GFP revealed a significant reduction in Vpr nuclear import and virion incorporation uniquely from the LTNP, whereas Vpr from the two progressing recipients displayed normal localisation and virion incorporation, implying a link between efficient Vpr nuclear import and HIV disease progression. Importantly, an F72L point mutation in the LTNP was identified for the first time as being uniquely responsible for decreased Vpr nuclear import.

KW - HIV (viruses)

KW - Viral Protein R

KW - epidemiology

KW - infection

KW - mutation

UR - http://handle.uws.edu.au:8081/1959.7/516056

U2 - 10.1186/1742-4690-5-67

DO - 10.1186/1742-4690-5-67

M3 - Article

C2 - 18638397

SN - 1742-4690

VL - 5

JO - Retrovirology

JF - Retrovirology

M1 - 67

ER -

Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor

Abstract

Open Access - Access Right Statement

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this