TY - JOUR
T1 - In vitro bioassay of Purpureocillium lilacinum and Bacillus thuringiensis for control of Meloidogyne incognita on black pepper (Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo
AU - Leong, Sui Sien
AU - Leong, Stephen Chan Teck
AU - Pau, Chen Guan
AU - Beattie, G. Andrew C.
PY - 2021
Y1 - 2021
N2 - This study aimed to evaluate strains of the entomopathogenic fungus Purpureocillium lilacinum and the bacterium Bacillus thuringiensis from Bintulu, in north-eastern Sarawak, Malaysia for their in vitro nematicidal properties against different developmental stages of Meloidogyne incognita. Ten indigenous novel strains of P. lilacinum, a commercial strain of the fungus (P. lilacinum M), and a strain of B. thuringiensis carrying Cry6 and Cry14 gene sequences were screened for parasitism against M. incognita females and eggs, egg hatching inhabitation and 2nd stage of active juveniles (J2) mortality. Our study demonstrates that P. lilacinum A and P. lilacinum B were the most effective biocontrol agent against M.incognita females, eggs and J2 due to highest infection of females, eggs and significant decrease in egg hatching events. P. lilacinum A, P. lilacinum B, and P. lilacinum M (positive control) demonstrated highly significant infection (>90%, P≤0.01) on M.incognita female nematodes. The present study revealed that spore suspension (105 spore/mL) of P. lilacinum A, P. lilacinum B and P. lilacinum M resulted in 78.8%, 66.0% and 73.4% parasitism on eggs, respectively. P. lilacinum A, P. lilacinum B and P. lilacinum M with low mortality of 6.0%, 5.5% and 5.7%, respectively, showed significant (P<0.05) differences in mortality effect on J2 of M.incognita as compared with control treatment (2.3%). Hatching of M. incognita eggs incubated in spore suspension of P. lilacinum A, P. lilacinum B and P. lilacinum M for seven days were reduced by 89% when corrected for control mortality (26%).
AB - This study aimed to evaluate strains of the entomopathogenic fungus Purpureocillium lilacinum and the bacterium Bacillus thuringiensis from Bintulu, in north-eastern Sarawak, Malaysia for their in vitro nematicidal properties against different developmental stages of Meloidogyne incognita. Ten indigenous novel strains of P. lilacinum, a commercial strain of the fungus (P. lilacinum M), and a strain of B. thuringiensis carrying Cry6 and Cry14 gene sequences were screened for parasitism against M. incognita females and eggs, egg hatching inhabitation and 2nd stage of active juveniles (J2) mortality. Our study demonstrates that P. lilacinum A and P. lilacinum B were the most effective biocontrol agent against M.incognita females, eggs and J2 due to highest infection of females, eggs and significant decrease in egg hatching events. P. lilacinum A, P. lilacinum B, and P. lilacinum M (positive control) demonstrated highly significant infection (>90%, P≤0.01) on M.incognita female nematodes. The present study revealed that spore suspension (105 spore/mL) of P. lilacinum A, P. lilacinum B and P. lilacinum M resulted in 78.8%, 66.0% and 73.4% parasitism on eggs, respectively. P. lilacinum A, P. lilacinum B and P. lilacinum M with low mortality of 6.0%, 5.5% and 5.7%, respectively, showed significant (P<0.05) differences in mortality effect on J2 of M.incognita as compared with control treatment (2.3%). Hatching of M. incognita eggs incubated in spore suspension of P. lilacinum A, P. lilacinum B and P. lilacinum M for seven days were reduced by 89% when corrected for control mortality (26%).
UR - https://hdl.handle.net/1959.7/uws:60846
UR - https://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=149520604&site=ehost-live&scope=site
U2 - 10.51963/jers.v23i1.1960
DO - 10.51963/jers.v23i1.1960
M3 - Article
SN - 1302-0250
VL - 23
SP - 41
EP - 59
JO - Journal of the Entomological Research Society
JF - Journal of the Entomological Research Society
IS - 1
ER -