Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)

Sarah B. Yakimowski; Paul D. Rymer; Harriet Stone; Spencer C. H. Barrett; Marcel E. Dorken

doi:10.1111/j.1755-0998.2008.02400.x

Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)

Sarah B. Yakimowski, Paul D. Rymer, Harriet Stone, Spencer C. H. Barrett, Marcel E. Dorken

Hawkesbury Institute for the Environment

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

We developed 11 microsatellite loci for Sagittaria latifolia, an aquatic plant common to wetlands of North America. From an (AG)-enriched library, we identified 66 unique microsatellite sequences for which primers could be designed. Twenty-two loci reliably amplified a clear single band of expected size, and 11 loci were scoreable and polymorphic. For these 11 loci, we genotyped a monoecious and a dioecious population, yielding four to 14 alleles per locus. Three loci exhibited significant linkage disequilibrium leaving eight independent variable loci. Eight loci also amplified in four other Sagittaria species. These microsatellite loci will be useful to compare genetic structure among monoecious and dioecious populations of S. latifolia.

Original language	English
Number of pages	3
Journal	Molecular Ecology Resources
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02400.x
Publication status	Published - 2009

Open Access - Access Right Statement

Keywords

Alismataceae
Sagittaria latifolia
botany
dioecy
microsatellites
monoecy

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10.1111/j.1755-0998.2008.02400.x

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@article{33460d9a889f4d41a47135d724c36100,

title = "Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)",

abstract = "We developed 11 microsatellite loci for Sagittaria latifolia, an aquatic plant common to wetlands of North America. From an (AG)-enriched library, we identified 66 unique microsatellite sequences for which primers could be designed. Twenty-two loci reliably amplified a clear single band of expected size, and 11 loci were scoreable and polymorphic. For these 11 loci, we genotyped a monoecious and a dioecious population, yielding four to 14 alleles per locus. Three loci exhibited significant linkage disequilibrium leaving eight independent variable loci. Eight loci also amplified in four other Sagittaria species. These microsatellite loci will be useful to compare genetic structure among monoecious and dioecious populations of S. latifolia.",

keywords = "Alismataceae, Sagittaria latifolia, botany, dioecy, microsatellites, monoecy",

author = "Yakimowski, {Sarah B.} and Rymer, {Paul D.} and Harriet Stone and Barrett, {Spencer C. H.} and Dorken, {Marcel E.}",

year = "2009",

doi = "10.1111/j.1755-0998.2008.02400.x",

language = "English",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

publisher = "Wiley-Blackwell Publishing Ltd",

}

TY - JOUR

T1 - Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)

AU - Yakimowski, Sarah B.

AU - Rymer, Paul D.

AU - Stone, Harriet

AU - Barrett, Spencer C. H.

AU - Dorken, Marcel E.

PY - 2009

Y1 - 2009

N2 - We developed 11 microsatellite loci for Sagittaria latifolia, an aquatic plant common to wetlands of North America. From an (AG)-enriched library, we identified 66 unique microsatellite sequences for which primers could be designed. Twenty-two loci reliably amplified a clear single band of expected size, and 11 loci were scoreable and polymorphic. For these 11 loci, we genotyped a monoecious and a dioecious population, yielding four to 14 alleles per locus. Three loci exhibited significant linkage disequilibrium leaving eight independent variable loci. Eight loci also amplified in four other Sagittaria species. These microsatellite loci will be useful to compare genetic structure among monoecious and dioecious populations of S. latifolia.

AB - We developed 11 microsatellite loci for Sagittaria latifolia, an aquatic plant common to wetlands of North America. From an (AG)-enriched library, we identified 66 unique microsatellite sequences for which primers could be designed. Twenty-two loci reliably amplified a clear single band of expected size, and 11 loci were scoreable and polymorphic. For these 11 loci, we genotyped a monoecious and a dioecious population, yielding four to 14 alleles per locus. Three loci exhibited significant linkage disequilibrium leaving eight independent variable loci. Eight loci also amplified in four other Sagittaria species. These microsatellite loci will be useful to compare genetic structure among monoecious and dioecious populations of S. latifolia.

KW - Alismataceae

KW - Sagittaria latifolia

KW - botany

KW - dioecy

KW - microsatellites

KW - monoecy

UR - http://handle.uws.edu.au:8081/1959.7/514938

U2 - 10.1111/j.1755-0998.2008.02400.x

DO - 10.1111/j.1755-0998.2008.02400.x

M3 - Article

SN - 1755-098X

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

ER -

Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)

Abstract

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Keywords

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