Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

Hannah McPherson; Carolyn Porter; Paul D. Rymer; Darren M. Crayn; Maurizio Rossetto

doi:10.1111/j.1755-0998.2008.02093.x

Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

Hannah McPherson, Carolyn Porter, Paul D. Rymer, Darren M. Crayn, Maurizio Rossetto

Research output: Contribution to journal › Article

Abstract

We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n-enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

Original language	English
Number of pages	3
Journal	Molecular Ecology Resources
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02093.x
Publication status	Published - 2008

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Keywords

Elaeocarpaceae
Tetratheca
Tremandraceae
botany
conservation
cross-transferability

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10.1111/j.1755-0998.2008.02093.x

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title = "Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)",

abstract = "We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n-enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.",

keywords = "Elaeocarpaceae, Tetratheca, Tremandraceae, botany, conservation, cross-transferability",

author = "Hannah McPherson and Carolyn Porter and Rymer, {Paul D.} and Crayn, {Darren M.} and Maurizio Rossetto",

year = "2008",

doi = "10.1111/j.1755-0998.2008.02093.x",

language = "English",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

AU - McPherson, Hannah

AU - Porter, Carolyn

AU - Rymer, Paul D.

AU - Crayn, Darren M.

AU - Rossetto, Maurizio

PY - 2008

Y1 - 2008

N2 - We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n-enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

AB - We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n-enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

KW - Elaeocarpaceae

KW - Tetratheca

KW - Tremandraceae

KW - botany

KW - conservation

KW - cross-transferability

UR - http://handle.uws.edu.au:8081/1959.7/514941

U2 - 10.1111/j.1755-0998.2008.02093.x

DO - 10.1111/j.1755-0998.2008.02093.x

M3 - Article

SN - 1755-098X

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

ER -

Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae)

Abstract

Open Access - Access Right Statement

Keywords

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