TY - JOUR
T1 - Isolation, expansion, and characterization of human islet-derived progenitor cells
AU - Joglekar, Mugdha V.
AU - Hardikar, Anandwardhan A.
PY - 2012
Y1 - 2012
N2 - Islet transplantation is a widely accepted and practiced cell replacement therapy for treatment of diabetes. However, scarcity of suitable cadaveric pancreas donors is a major limitation that restricts the availability of this therapy to millions of diabetic individuals worldwide. Research in the field has therefore focused on search for an alternate cell source. Various stem/progenitor cells have been considered to be suitable for replacement therapy in diabetes since they have the potential to proliferate and differentiate. Over last few years, we have specifically focused our attention on understanding the potential of progenitor cells that are derived from in vitro expansion of human islets. Since epigenetic marks that define an "active" insulin promoter region in beta cells are inherited during in vitro expansion, we believe that human islet-derived progenitor cells (hIPCs) represent a lineage-committed population of islet precursor cells. Here, we describe details of the method for isolation, expansion, and characterization of human islet-derived progenitor cells (hIPCs).
AB - Islet transplantation is a widely accepted and practiced cell replacement therapy for treatment of diabetes. However, scarcity of suitable cadaveric pancreas donors is a major limitation that restricts the availability of this therapy to millions of diabetic individuals worldwide. Research in the field has therefore focused on search for an alternate cell source. Various stem/progenitor cells have been considered to be suitable for replacement therapy in diabetes since they have the potential to proliferate and differentiate. Over last few years, we have specifically focused our attention on understanding the potential of progenitor cells that are derived from in vitro expansion of human islets. Since epigenetic marks that define an "active" insulin promoter region in beta cells are inherited during in vitro expansion, we believe that human islet-derived progenitor cells (hIPCs) represent a lineage-committed population of islet precursor cells. Here, we describe details of the method for isolation, expansion, and characterization of human islet-derived progenitor cells (hIPCs).
KW - Chromatin immunoprecipitation
KW - Islet
KW - Isolation
KW - Pancreas
KW - Progenitor cells
UR - http://www.scopus.com/inward/record.url?scp=84861837627&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-815-3_21
DO - 10.1007/978-1-61779-815-3_21
M3 - Article
C2 - 22610570
AN - SCOPUS:84861837627
SN - 1064-3745
VL - 879
SP - 351
EP - 366
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -
