Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus

R. Patel; W. L. Chan; L. M. Kemp; N. B.La Thangue; D. S. Latchman

doi:10.1093/nar/14.14.5629

Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus

R. Patel, W. L. Chan, L. M. Kemp, N. B.La Thangue, D. S. Latchman

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

A small number of cellular proteins accumulate to high levels in cells infected with Herpes Simplex Virus (HSV) despite a generalised repression of most host cell bio-synthesis. An antibody to one such protein has been used to screen a lambda gtll library and far polysome immunoprecipitation in order to isolate cDNA clones derived from the corresponding gene. The cDNA clones have been used in dot blot and nuclear run-off assays to show that HSV, like other DNA tumour viruses can transcriptionally induce a cellular gene. The mechanism of this effect which is dependent on viral protein synthesis and its possible significance in transformation by HSV are discussed.

Original language	English
Pages (from-to)	5629-5640
Number of pages	12
Journal	Nucleic Acids Research
Volume	14
Issue number	14
DOIs	https://doi.org/10.1093/nar/14.14.5629
Publication status	Published - 11 Jul 1986
Externally published	Yes

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Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus

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