TY - JOUR
T1 - Isolation of the active constituents in natural materials by 'heart cutting' isocratic reversed-phase two-dimensional liquid chromatography
AU - Wong, Victor
AU - Shalliker, Ross A.
PY - 2004
Y1 - 2004
N2 - A multidimensional heart-cutting reversed-phase HPLC separation approach, where two columns were operated independently via two six-port two-position switching valves, was employed in the isolation of a major bioactive found in the ethanol–water (80:20) crude extract of Clerodendrum floribundum. In this mode of operation, the specific productivity of the multidimensional approach under overload conditions was twice that of conventional gradient methods with the same loading factor. Isolated sample purities were greater than 99% with recoveries of 95%. The independent operation of each of the two columns employed in the multidimensional approach allowed the cycle time to be less than 7ÃÂ min, compared to 23ÃÂ min in the gradient elution single-dimension mode of operation.
AB - A multidimensional heart-cutting reversed-phase HPLC separation approach, where two columns were operated independently via two six-port two-position switching valves, was employed in the isolation of a major bioactive found in the ethanol–water (80:20) crude extract of Clerodendrum floribundum. In this mode of operation, the specific productivity of the multidimensional approach under overload conditions was twice that of conventional gradient methods with the same loading factor. Isolated sample purities were greater than 99% with recoveries of 95%. The independent operation of each of the two columns employed in the multidimensional approach allowed the cycle time to be less than 7ÃÂ min, compared to 23ÃÂ min in the gradient elution single-dimension mode of operation.
KW - Liquid chromatography
KW - Multidimensional chromatography
KW - Separation (Technology)
UR - http://handle.uws.edu.au:8081/1959.7/9733
U2 - 10.1016/j.chroma.2004.02.003
DO - 10.1016/j.chroma.2004.02.003
M3 - Article
SN - 0021-9673
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -