LC coupled with TOFMS for metabonomics study of mini-pigs with atherosclerosis

Ming-Qian Sun, Jian-Xun Liu, Lan Miao, Jin Cao, Cheng-Ren Lin, Lei Li, Jianxun Ren

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

In this study, serum metabolic profiles of mini-pigs with atherosclerosis (AS)were analyzed by LC–TOFMS. Partial least-squares to latent structure-discriminant analysis and orthogonal projection to latent structure-discriminant analysis were used for group differentiation and selection of potential biomarkers. The mini-pig disease models were constructed by feeding a high-fat diet and inducing coronary injury, in accordance with the mechanism of AS pathogenesis. To characterize the development of AS, serum samples were collected and analyzed at two time points (two and ten weeks). Separate distinct clustering of results from normal and model mini-pigs could be observed for both the two and ten-week samples. With the development of AS, the metabolism of the model mini-pigs was more substantially disturbed. Major metabolites contributing to the discrimination were fatty acids, lysophosphatidylcholines, and bile acids. These potential biomarkers are related with inflammation, oxidative stress, and abnormal lipid and energy metabolism.
Original languageEnglish
Pages (from-to)491-497
Number of pages7
JournalChromatographia
Volume75
Issue number45574
DOIs
Publication statusPublished - 2012

Keywords

  • atherosclerosis
  • biochemical markers
  • liquid chromatography
  • mass spectrometry
  • miniature pigs

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