LongSAGE profiling of nine human embryonic stem cell lines

Martin Hirst; Allen D. Delaney; Sean A. Rogers; Angelique Schnerch; Deryck R. Persaud; Michael D. O&apos;Connor; Thomas Zeng; Michelle Moksa; Keith Fichter; Diana Mah; Anne Go; Ryan D. Morin; Agnes Baross; Yongjun Zhao; Jaswinder Khattra; Anna-Liisa Prabhu; Pawan Pandoh; Helen L. McDonald; Jennifer Asano; Noreen Dhalla; Kevin Ma; Stephanie Lee; Adrian Ally; Neil Chahal; Stephanie Menzies; Asim Siddiqui; Robert Holt; Steven Jones; Daniela S. Gerhard; James A. Thomson; Connie J. Eaves; Marco A. Marra

LongSAGE profiling of nine human embryonic stem cell lines

Martin Hirst
, Allen D. Delaney
, Sean A. Rogers
, Angelique Schnerch
, Deryck R. Persaud
, Michael D. O'Connor
, Thomas Zeng
, Michelle Moksa
, Keith Fichter
, Diana Mah
, Anne Go
, Ryan D. Morin
, Agnes Baross
, Yongjun Zhao
, Jaswinder Khattra
, Anna-Liisa Prabhu
, Pawan Pandoh
, Helen L. McDonald
, Jennifer Asano
, Noreen Dhalla

Kevin Ma, Stephanie Lee, Adrian Ally, Neil Chahal, Stephanie Menzies, Asim Siddiqui, Robert Holt, Steven Jones, Daniela S. Gerhard, James A. Thomson, Connie J. Eaves, Marco A. Marra

School of Medicine

Research output: Contribution to journal › Article

20 Citations (Scopus)

Abstract

To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.

Original language	English
Number of pages	12
Journal	Genome Biology
Volume	8
Issue number	6
Publication status	Published - 2007

Open Access - Access Right Statement

© 2007 Hirst et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

blastocysts
embryo
human embryonic stem cell
propagation
proteins
stem cells

Cite this

@article{fb3913d626e04456814e456c2544c2ca,

title = "LongSAGE profiling of nine human embryonic stem cell lines",

abstract = "To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.",

keywords = "blastocysts, embryo, human embryonic stem cell, propagation, proteins, stem cells",

author = "Martin Hirst and Delaney, \{Allen D.\} and Rogers, \{Sean A.\} and Angelique Schnerch and Persaud, \{Deryck R.\} and O\'Connor, \{Michael D.\} and Thomas Zeng and Michelle Moksa and Keith Fichter and Diana Mah and Anne Go and Morin, \{Ryan D.\} and Agnes Baross and Yongjun Zhao and Jaswinder Khattra and Anna-Liisa Prabhu and Pawan Pandoh and McDonald, \{Helen L.\} and Jennifer Asano and Noreen Dhalla and Kevin Ma and Stephanie Lee and Adrian Ally and Neil Chahal and Stephanie Menzies and Asim Siddiqui and Robert Holt and Steven Jones and Gerhard, \{Daniela S.\} and Thomson, \{James A.\} and Eaves, \{Connie J.\} and Marra, \{Marco A.\}",

year = "2007",

language = "English",

volume = "8",

journal = "Genome Biology",

issn = "1465-6906",

publisher = "BioMed Central",

number = "6",

}

Hirst, M, Delaney, AD, Rogers, SA, Schnerch, A, Persaud, DR, O'Connor, MD, Zeng, T, Moksa, M, Fichter, K, Mah, D, Go, A, Morin, RD, Baross, A, Zhao, Y, Khattra, J, Prabhu, A-L, Pandoh, P, McDonald, HL, Asano, J, Dhalla, N, Ma, K, Lee, S, Ally, A, Chahal, N, Menzies, S, Siddiqui, A, Holt, R, Jones, S, Gerhard, DS, Thomson, JA, Eaves, CJ & Marra, MA 2007, 'LongSAGE profiling of nine human embryonic stem cell lines', Genome Biology, vol. 8, no. 6.

TY - JOUR

T1 - LongSAGE profiling of nine human embryonic stem cell lines

AU - Hirst, Martin

AU - Delaney, Allen D.

AU - Rogers, Sean A.

AU - Schnerch, Angelique

AU - Persaud, Deryck R.

AU - O'Connor, Michael D.

AU - Zeng, Thomas

AU - Moksa, Michelle

AU - Fichter, Keith

AU - Mah, Diana

AU - Go, Anne

AU - Morin, Ryan D.

AU - Baross, Agnes

AU - Zhao, Yongjun

AU - Khattra, Jaswinder

AU - Prabhu, Anna-Liisa

AU - Pandoh, Pawan

AU - McDonald, Helen L.

AU - Asano, Jennifer

AU - Dhalla, Noreen

AU - Ma, Kevin

AU - Lee, Stephanie

AU - Ally, Adrian

AU - Chahal, Neil

AU - Menzies, Stephanie

AU - Siddiqui, Asim

AU - Holt, Robert

AU - Jones, Steven

AU - Gerhard, Daniela S.

AU - Thomson, James A.

AU - Eaves, Connie J.

AU - Marra, Marco A.

PY - 2007

Y1 - 2007

N2 - To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.

AB - To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.

KW - blastocysts

KW - embryo

KW - human embryonic stem cell

KW - propagation

KW - proteins

KW - stem cells

UR - http://handle.uws.edu.au:8081/1959.7/515295

UR - http://www.genomebiology.com/2007/8/6/R11310.1186/gb-2007-8-6-r113

M3 - Article

C2 - 17570852

SN - 1465-6906

VL - 8

JO - Genome Biology

JF - Genome Biology

IS - 6

ER -

LongSAGE profiling of nine human embryonic stem cell lines

Abstract

Open Access - Access Right Statement

Keywords

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