Lysozyme depolymerization of photo-activated chitosan adhesive films

Damia Mawad; Charles Warren; Mathew Barton; David Mahns; John Morley; Binh T. T. Pham; Nguyen T. H. Pham; Sindy Kueh; Antonio Lauto

doi:10.1016/j.carbpol.2014.12.008

Lysozyme depolymerization of photo-activated chitosan adhesive films

Damia Mawad, Charles Warren, Mathew Barton, David Mahns, John Morley, Binh T. T. Pham, Nguyen T. H. Pham, Sindy Kueh, Antonio Lauto

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Effective tissue bioadhesion of rose bengal-chitosan films can be achieved by photoactivation using a green laser. In this study, lysozyme was incorporated in these films to enhance the rate of depolymerization and assess the laser impact on lysozyme. The lysozyme loaded films exhibited a 21% mass loss after 4 weeks implantation in rats while control films (without lysozyme) had only 7% mass loss. Capillary electrophoresis-mass spectroscopy showed that chitosan degraded into monomers and oligomers of glucosamine and N-acetyl-glucosamine. Irradiation with laser did not affect the depolymerization of adhesive by lysozyme suggesting that the inclusion of lysozyme in the bioadhesive is a viable technique for tailoring the depolymerization.

Original language	English
Pages (from-to)	56-63
Number of pages	8
Journal	Carbohydrate Polymers
Volume	121
DOIs	https://doi.org/10.1016/j.carbpol.2014.12.008
Publication status	Published - 2015

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10.1016/j.carbpol.2014.12.008

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@article{4d7142eab9884a79b101953e60c2586b,

title = "Lysozyme depolymerization of photo-activated chitosan adhesive films",

abstract = "Effective tissue bioadhesion of rose bengal-chitosan films can be achieved by photoactivation using a green laser. In this study, lysozyme was incorporated in these films to enhance the rate of depolymerization and assess the laser impact on lysozyme. The lysozyme loaded films exhibited a 21% mass loss after 4 weeks implantation in rats while control films (without lysozyme) had only 7% mass loss. Capillary electrophoresis-mass spectroscopy showed that chitosan degraded into monomers and oligomers of glucosamine and N-acetyl-glucosamine. Irradiation with laser did not affect the depolymerization of adhesive by lysozyme suggesting that the inclusion of lysozyme in the bioadhesive is a viable technique for tailoring the depolymerization.",

author = "Damia Mawad and Charles Warren and Mathew Barton and David Mahns and John Morley and Pham, {Binh T. T.} and Pham, {Nguyen T. H.} and Sindy Kueh and Antonio Lauto",

year = "2015",

doi = "10.1016/j.carbpol.2014.12.008",

language = "English",

volume = "121",

pages = "56--63",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier",

}

TY - JOUR

T1 - Lysozyme depolymerization of photo-activated chitosan adhesive films

AU - Mawad, Damia

AU - Warren, Charles

AU - Barton, Mathew

AU - Mahns, David

AU - Morley, John

AU - Pham, Binh T. T.

AU - Pham, Nguyen T. H.

AU - Kueh, Sindy

AU - Lauto, Antonio

PY - 2015

Y1 - 2015

N2 - Effective tissue bioadhesion of rose bengal-chitosan films can be achieved by photoactivation using a green laser. In this study, lysozyme was incorporated in these films to enhance the rate of depolymerization and assess the laser impact on lysozyme. The lysozyme loaded films exhibited a 21% mass loss after 4 weeks implantation in rats while control films (without lysozyme) had only 7% mass loss. Capillary electrophoresis-mass spectroscopy showed that chitosan degraded into monomers and oligomers of glucosamine and N-acetyl-glucosamine. Irradiation with laser did not affect the depolymerization of adhesive by lysozyme suggesting that the inclusion of lysozyme in the bioadhesive is a viable technique for tailoring the depolymerization.

AB - Effective tissue bioadhesion of rose bengal-chitosan films can be achieved by photoactivation using a green laser. In this study, lysozyme was incorporated in these films to enhance the rate of depolymerization and assess the laser impact on lysozyme. The lysozyme loaded films exhibited a 21% mass loss after 4 weeks implantation in rats while control films (without lysozyme) had only 7% mass loss. Capillary electrophoresis-mass spectroscopy showed that chitosan degraded into monomers and oligomers of glucosamine and N-acetyl-glucosamine. Irradiation with laser did not affect the depolymerization of adhesive by lysozyme suggesting that the inclusion of lysozyme in the bioadhesive is a viable technique for tailoring the depolymerization.

UR - http://handle.uws.edu.au:8081/1959.7/563962

U2 - 10.1016/j.carbpol.2014.12.008

DO - 10.1016/j.carbpol.2014.12.008

M3 - Article

C2 - 25659671

SN - 0144-8617

VL - 121

SP - 56

EP - 63

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Lysozyme depolymerization of photo-activated chitosan adhesive films

Abstract

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