TY - JOUR
T1 - Measurements of stem xylem hydraulic conductivity in the laboratory and field
AU - Melcher, Peter J.
AU - Holbrook, N. Michele
AU - Burns, Michael J.
AU - Zwieniecki, Maciej A.
AU - Cobb, Alexander R.
AU - Brodribb, Timothy J.
AU - Choat, Brendan
AU - Sack, Lawren
PY - 2012
Y1 - 2012
N2 - 1. Xylem hydraulic properties play an essential role in supporting growth and photosynthesis and influence sensitivity to environmental conditions such as drought and freezing. Consequently, stem hydraulic conductance can be used as a comparative measure of overall hydraulic adaptation across species and to assess the impact of environmental variation, especially drought, on water transport. 2. We summarize the main methods currently in use for measurements of stem xylem hydraulic properties. Measurements can be accomplished in a number of ways, including using a pipette, an analytic balance or a 'pressure-drop' flow meter. We provide new details on the design of a relatively inexpensive and easily field-deployable flow meter that is flexible for a variety of applications. The biological challenges associated with these measurements arise from the difficulties of working with diverse living tissues of variable geometry. 3. We provide a review of best practices and provide technical guidance, emphasizing measurements on detached samples using portable equipment.
AB - 1. Xylem hydraulic properties play an essential role in supporting growth and photosynthesis and influence sensitivity to environmental conditions such as drought and freezing. Consequently, stem hydraulic conductance can be used as a comparative measure of overall hydraulic adaptation across species and to assess the impact of environmental variation, especially drought, on water transport. 2. We summarize the main methods currently in use for measurements of stem xylem hydraulic properties. Measurements can be accomplished in a number of ways, including using a pipette, an analytic balance or a 'pressure-drop' flow meter. We provide new details on the design of a relatively inexpensive and easily field-deployable flow meter that is flexible for a variety of applications. The biological challenges associated with these measurements arise from the difficulties of working with diverse living tissues of variable geometry. 3. We provide a review of best practices and provide technical guidance, emphasizing measurements on detached samples using portable equipment.
KW - cavitation
KW - embolism
KW - flow meters
KW - hydraulic conductivity
KW - xylem
UR - http://handle.uws.edu.au:8081/1959.7/522249
U2 - 10.1111/j.2041-210X.2012.00204.x
DO - 10.1111/j.2041-210X.2012.00204.x
M3 - Article
SN - 2041-210X
VL - 3
SP - 685
EP - 694
JO - Methods in Ecology and Evolution
JF - Methods in Ecology and Evolution
IS - 4
ER -