TY - JOUR
T1 - Mechanisms of pollution induced community tolerance in a soil microbial community exposed to Cu
AU - Wakelin, Steven
AU - Gerard, Emily
AU - Black, Amanda
AU - Hamonts, Kelly
AU - Condron, Leo
AU - Yuan, Tong
AU - Van Nostrand, Joy
AU - Zhou, Jizhong
AU - O’Callaghan, Maureen
PY - 2014
Y1 - 2014
N2 - Pollution induced community tolerance (PICT) to Cu2+, and co-tolerance to nanoparticulate Cu, ionic silver (Ag+), and vancomycin were measured in field soils treated with Cu2+ 15 years previously. EC50 values were determined using substrate induced respiration and correlations made against soil physicochemical properties, microbial community structure, physiological status (qCO2; metabolic quotient), and abundances of genes associated with metal and antibiotic resistance. Previous level of exposure to copper was directly (P < 0.05) associated with tolerance to addition of new Cu2+, and also of nanoparticle Cu. However, Cu-exposed communities had no co-tolerance to Ag+ and had increased susceptibly to vancomycin. Increased tolerance to both Cu correlated (P < 0.05) with increased metabolic quotient, potentially indicating that the community directed more energy towards cellular maintenance rather than biomass production. Neither bacterial or fungal community composition nor changes in the abundance of genes involved with metal resistance were related to PICT or co-tolerance mechanisms.
AB - Pollution induced community tolerance (PICT) to Cu2+, and co-tolerance to nanoparticulate Cu, ionic silver (Ag+), and vancomycin were measured in field soils treated with Cu2+ 15 years previously. EC50 values were determined using substrate induced respiration and correlations made against soil physicochemical properties, microbial community structure, physiological status (qCO2; metabolic quotient), and abundances of genes associated with metal and antibiotic resistance. Previous level of exposure to copper was directly (P < 0.05) associated with tolerance to addition of new Cu2+, and also of nanoparticle Cu. However, Cu-exposed communities had no co-tolerance to Ag+ and had increased susceptibly to vancomycin. Increased tolerance to both Cu correlated (P < 0.05) with increased metabolic quotient, potentially indicating that the community directed more energy towards cellular maintenance rather than biomass production. Neither bacterial or fungal community composition nor changes in the abundance of genes involved with metal resistance were related to PICT or co-tolerance mechanisms.
UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:41696
U2 - 10.1016/j.envpol.2014.03.008
DO - 10.1016/j.envpol.2014.03.008
M3 - Article
SN - 0269-7491
VL - 190
SP - 1
EP - 9
JO - Environmental Pollution
JF - Environmental Pollution
ER -