Metabonomics study of heart homogenates from myocardial infarction rats using liquid chromatography/time of flight mass spectrometry

Mingqian Sun; Lei Sun; Lan Miao; Li Lin; Shuo Huang; Bin Yang; Jianhua Fu; Zhengyan Ge; Long Jin; Jianxun Liu

doi:10.1007/s10337-016-3136-7

Metabonomics study of heart homogenates from myocardial infarction rats using liquid chromatography/time of flight mass spectrometry

Mingqian Sun, Lei Sun, Lan Miao, Li Lin, Shuo Huang, Bin Yang, Jianhua Fu, Zhengyan Ge, Long Jin, Jianxun Liu

Western Sydney University

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, a metabonomics analysis of heart homogenates from myocardial ischemic rats was performed by LC–TOF–MS. Hydrophilic interaction chromatography (HILIC) was used to separate the endogenous metabolites in heart homogenates. Partial least squares to latent structure-discriminant analysis (PLS-DA) was used for data analysis. Good separations were observed between the normal and model groups and 15 potential biomarkers were identified. The major disturbed metabolic pathways were purine metabolism, pyrimidine metabolism, urea cycle, and energy metabolism. The results demonstrated that a metabonomics approach based on HILIC-MS was useful for studying metabolic mechanism on target tissue of the myocardial infarction rat.

Original language	English
Pages (from-to)	1309-1316
Number of pages	8
Journal	Chromatographia
Volume	79
Issue number	19-20
DOIs	https://doi.org/10.1007/s10337-016-3136-7
Publication status	Published - 2016

Keywords

biochemical markers
liquid chromatography
mass spectrometry
microbial metabolites
myocardial infarction

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10.1007/s10337-016-3136-7

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title = "Metabonomics study of heart homogenates from myocardial infarction rats using liquid chromatography/time of flight mass spectrometry",

abstract = "In this study, a metabonomics analysis of heart homogenates from myocardial ischemic rats was performed by LC–TOF–MS. Hydrophilic interaction chromatography (HILIC) was used to separate the endogenous metabolites in heart homogenates. Partial least squares to latent structure-discriminant analysis (PLS-DA) was used for data analysis. Good separations were observed between the normal and model groups and 15 potential biomarkers were identified. The major disturbed metabolic pathways were purine metabolism, pyrimidine metabolism, urea cycle, and energy metabolism. The results demonstrated that a metabonomics approach based on HILIC-MS was useful for studying metabolic mechanism on target tissue of the myocardial infarction rat.",

keywords = "biochemical markers, liquid chromatography, mass spectrometry, microbial metabolites, myocardial infarction",

author = "Mingqian Sun and Lei Sun and Lan Miao and Li Lin and Shuo Huang and Bin Yang and Jianhua Fu and Zhengyan Ge and Long Jin and Jianxun Liu",

year = "2016",

doi = "10.1007/s10337-016-3136-7",

language = "English",

volume = "79",

pages = "1309--1316",

journal = "Chromatographia",

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TY - JOUR

T1 - Metabonomics study of heart homogenates from myocardial infarction rats using liquid chromatography/time of flight mass spectrometry

AU - Sun, Mingqian

AU - Sun, Lei

AU - Miao, Lan

AU - Lin, Li

AU - Huang, Shuo

AU - Yang, Bin

AU - Fu, Jianhua

AU - Ge, Zhengyan

AU - Jin, Long

AU - Liu, Jianxun

PY - 2016

Y1 - 2016

N2 - In this study, a metabonomics analysis of heart homogenates from myocardial ischemic rats was performed by LC–TOF–MS. Hydrophilic interaction chromatography (HILIC) was used to separate the endogenous metabolites in heart homogenates. Partial least squares to latent structure-discriminant analysis (PLS-DA) was used for data analysis. Good separations were observed between the normal and model groups and 15 potential biomarkers were identified. The major disturbed metabolic pathways were purine metabolism, pyrimidine metabolism, urea cycle, and energy metabolism. The results demonstrated that a metabonomics approach based on HILIC-MS was useful for studying metabolic mechanism on target tissue of the myocardial infarction rat.

AB - In this study, a metabonomics analysis of heart homogenates from myocardial ischemic rats was performed by LC–TOF–MS. Hydrophilic interaction chromatography (HILIC) was used to separate the endogenous metabolites in heart homogenates. Partial least squares to latent structure-discriminant analysis (PLS-DA) was used for data analysis. Good separations were observed between the normal and model groups and 15 potential biomarkers were identified. The major disturbed metabolic pathways were purine metabolism, pyrimidine metabolism, urea cycle, and energy metabolism. The results demonstrated that a metabonomics approach based on HILIC-MS was useful for studying metabolic mechanism on target tissue of the myocardial infarction rat.

KW - biochemical markers

KW - liquid chromatography

KW - mass spectrometry

KW - microbial metabolites

KW - myocardial infarction

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:42992

U2 - 10.1007/s10337-016-3136-7

DO - 10.1007/s10337-016-3136-7

M3 - Article

SN - 0009-5893

VL - 79

SP - 1309

EP - 1316

JO - Chromatographia

JF - Chromatographia

IS - 19-20

ER -

Metabonomics study of heart homogenates from myocardial infarction rats using liquid chromatography/time of flight mass spectrometry

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