TY - JOUR
T1 - Microbial community structure analysis and isolation of vanadium-resistant strains in vanadium mining-impacted soil
AU - Lu, L.
AU - Li, J.
AU - Hu, A.
AU - Mulla, S. I.
AU - Yang, J.
AU - Yu, C.- P.
PY - 2019
Y1 - 2019
N2 - Vanadium (V) is an essential trace metal for living organisms with broad application, and extensive mining activities have caused heavy metal pollution in the mining area. Currently, there is little information available on the microbial ecology of V mining-impacted soil. Here we report the analysis of microbial community and diversity of V-resistant bacteria in the V-titanium (Ti) magnetite (Fe3O4) mining area in Panzhihua, southwestern China, which is one of the biggestV-Ti Fe3O4 production bases in the world.We found thatV made the key contribution to the distribution pattern and microbial community of sampling sites. The sequencing analysis showed high diversity in both surface and subsurface soil samples; in total 129 V-resistant strains (84 strains with V5+-resistance and 45 strains V4+-resistance) were isolated from the metal contaminated soil samples. Actinobacteria, Proteobacteria, and Firmicutes were found to be the dominant phyla under both of the cultivation-dependent and cultivation-independent approaches. Arthrobacter, Streptomyces, Rhodococcus, Nocardioides, Paenibacillus, Bacillus, and Burkholderia were the dominant V-resistant genera, and their tolerance to V varied among different strains. Arthrobacter was found to be the most dominant genera among the isolated strains. Eight isolates with better resistance to V also showed resistance to more than nine heavy metals as well as several antibiotics.The phylogenetically diverse metal-resistant strains could be potential resources for bioremediation of metal-contaminated soils.
AB - Vanadium (V) is an essential trace metal for living organisms with broad application, and extensive mining activities have caused heavy metal pollution in the mining area. Currently, there is little information available on the microbial ecology of V mining-impacted soil. Here we report the analysis of microbial community and diversity of V-resistant bacteria in the V-titanium (Ti) magnetite (Fe3O4) mining area in Panzhihua, southwestern China, which is one of the biggestV-Ti Fe3O4 production bases in the world.We found thatV made the key contribution to the distribution pattern and microbial community of sampling sites. The sequencing analysis showed high diversity in both surface and subsurface soil samples; in total 129 V-resistant strains (84 strains with V5+-resistance and 45 strains V4+-resistance) were isolated from the metal contaminated soil samples. Actinobacteria, Proteobacteria, and Firmicutes were found to be the dominant phyla under both of the cultivation-dependent and cultivation-independent approaches. Arthrobacter, Streptomyces, Rhodococcus, Nocardioides, Paenibacillus, Bacillus, and Burkholderia were the dominant V-resistant genera, and their tolerance to V varied among different strains. Arthrobacter was found to be the most dominant genera among the isolated strains. Eight isolates with better resistance to V also showed resistance to more than nine heavy metals as well as several antibiotics.The phylogenetically diverse metal-resistant strains could be potential resources for bioremediation of metal-contaminated soils.
UR - https://hdl.handle.net/1959.7/uws:64558
U2 - 10.2489/jswc.74.3.296
DO - 10.2489/jswc.74.3.296
M3 - Article
SN - 0022-4561
VL - 74
SP - 296
EP - 308
JO - Journal of Soil and Water Conservation
JF - Journal of Soil and Water Conservation
IS - 3
ER -
