Abstract
Many plants of commercial interest are propagated in tissue culture, with some presenting difficulties in their mineral balance. Historically, this issue has been addressed by varying the mineral composition of the media hoping to find a satisfactory combination. However, a potentially more efficient approach has been successful for several species, i.e., amendment of the media composition based on the mineral content of the plantlet leaves relative to that of the young leaves of thriving, field-grown plants. We used the latter approach to address a mineral imbalance encountered in propagating the Australian native plant, spotted iron gum (Corymbia maculata (syn. Eucalyptus maculata)). The chemical analysis showed that the concentration of iron in the plantlets was far in excess of requirement, and the plantlets thrived when its concentration in the medium was decreased 10-fold. This success supports the proposition that the mineral analysis of plantlets, together with the published data on foliar sufficiency ranges, may provide an efficient, general approach to optimizing the mineral composition of media for the in vitro production of plants.
| Original language | English |
|---|---|
| Pages (from-to) | 844-846 |
| Number of pages | 3 |
| Journal | In Vitro Cellular and Developmental Biology: Plant |
| Volume | 59 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2023 |
Bibliographical note
Publisher Copyright:© 2023, The Author(s).
Open Access - Access Right Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Keywords
- Mineral sufficiency levels
- Unthrifty in vitro plantlets