Moderate heat stress prevented the observed biomass and yield stimulation caused by elevated CO2 in two well-watered wheat cultivars

Key message: Heat stress (HS) under well-watered conditions was not detrimental to leaf photosynthesis or yield but modified the elevated CO₂ response of photosynthesis and yield in two contrasting wheat cultivars. Abstract: Climate change is increasing the frequency of extreme events such as heat waves, adversely affecting crop productivity. While positive impacts of elevated carbon dioxide (eCO₂) on crop productivity are evident, the interactive effects of eCO₂ and environmental stresses are still unclear. To investigate the interactive effects of elevated CO₂ and heat stress (HS), we grew two contrasting wheat cultivars, early-maturing Scout and high-tillering Yitpi, under non-limiting water and nutrients at ambient (aCO₂, 450 ppm) or elevated (eCO₂, 650 ppm) CO₂ and 22 °C in the glasshouse. Plants were exposed to two 3-day HS cycles at the vegetative (38.1 °C) and/or flowering (33.5 °C) stage. At aCO₂, both wheat cultivars showed similar responses of photosynthesis and mesophyll conductance to temperature and produced similar grain yield. Relative to aCO₂, eCO₂ enhanced photosynthesis rate and reduced stomatal conductance and maximal carboxylation rate (V_cmax). During HS, high temperature stimulated photosynthesis at eCO₂ in both cultivars, while eCO₂ stimulated photosynthesis in Scout. Electron transport rate (J_max) was unaffected by any treatment. eCO₂ equally enhanced biomass and grain yield of both cultivars in control, but not HS, plants. HS reduced biomass and yield of Scout at eCO₂. Yitpi, the cultivar with higher grain nitrogen, underwent a trade-off between grain yield and nitrogen. In conclusion, eCO₂ improved photosynthesis of control and HS wheat, and improved biomass and grain yield of control plants only. Under well-watered conditions, HS was not detrimental to photosynthesis or growth but precluded a yield response to eCO₂.