Molecular evolution and functional modification of plant miRNAs with CRISPR

Fenglin Deng; Fanrong Zeng; Qiufang Shen; Asad Abbas; Jianhui Cheng; Wei Jiang; Guang Chen; Adnan Noor Shah; Paul Holford; Mohsin Tanveer; Dabing Zhang; Zhong-Hua Chen

Molecular evolution and functional modification of plant miRNAs with CRISPR

Fenglin Deng, Fanrong Zeng, Qiufang Shen, Asad Abbas, Jianhui Cheng, Wei Jiang, Guang Chen, Adnan Noor Shah, Paul Holford, Mohsin Tanveer, Dabing Zhang, Zhong-Hua Chen

Western Sydney University

Research output: Contribution to journal › Article › peer-review

Abstract

Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.

Original language	English
Pages (from-to)	890-907
Number of pages	18
Journal	Trends in Plant Science
Volume	27
Issue number	9
Publication status	Published - 2022

Cite this

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title = "Molecular evolution and functional modification of plant miRNAs with CRISPR",

abstract = "Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.",

author = "Fenglin Deng and Fanrong Zeng and Qiufang Shen and Asad Abbas and Jianhui Cheng and Wei Jiang and Guang Chen and Shah, {Adnan Noor} and Paul Holford and Mohsin Tanveer and Dabing Zhang and Zhong-Hua Chen",

year = "2022",

language = "English",

volume = "27",

pages = "890--907",

journal = "Trends in Plant Science",

issn = "1360-1385",

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TY - JOUR

T1 - Molecular evolution and functional modification of plant miRNAs with CRISPR

AU - Deng, Fenglin

AU - Zeng, Fanrong

AU - Shen, Qiufang

AU - Abbas, Asad

AU - Cheng, Jianhui

AU - Jiang, Wei

AU - Chen, Guang

AU - Shah, Adnan Noor

AU - Holford, Paul

AU - Tanveer, Mohsin

AU - Zhang, Dabing

AU - Chen, Zhong-Hua

PY - 2022

Y1 - 2022

N2 - Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.

AB - Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.

UR - https://hdl.handle.net/1959.7/uws:67813

M3 - Article

SN - 1360-1385

VL - 27

SP - 890

EP - 907

JO - Trends in Plant Science

JF - Trends in Plant Science

IS - 9

ER -

Molecular evolution and functional modification of plant miRNAs with CRISPR

Abstract

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