Multiplexed Detection

Sercan Pravadali-Cekic; Danijela Kocic; Xavier Conlan; R. Andrew Shalliker

doi:10.1080/10826076.2015.1105256

Multiplexed Detection

Sercan Pravadali-Cekic, Danijela Kocic, Xavier Conlan, R. Andrew Shalliker

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH"¢ for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.

Original language	English
Pages (from-to)	1753-1758
Number of pages	6
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	38
Issue number	19
DOIs	https://doi.org/10.1080/10826076.2015.1105256
Publication status	Published - 26 Nov 2015

Bibliographical note

Publisher Copyright:
© 2015 Taylor & Francis Group, LLC.

Keywords

chemistry, analytic
high performance liquid chromatography
multiplexing
tobacco

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10.1080/10826076.2015.1105256

Cite this

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title = "Multiplexed Detection",

abstract = "Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH{"}¢ for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.",

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TY - JOUR

T1 - Multiplexed Detection

AU - Pravadali-Cekic, Sercan

AU - Kocic, Danijela

AU - Conlan, Xavier

AU - Shalliker, R. Andrew

PY - 2015/11/26

Y1 - 2015/11/26

N2 - Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH"¢ for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.

AB - Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH"¢ for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.

KW - chemistry, analytic

KW - high performance liquid chromatography

KW - multiplexing

KW - tobacco

UR - http://handle.uws.edu.au:8081/1959.7/uws:32990

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DO - 10.1080/10826076.2015.1105256

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EP - 1758

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

IS - 19

ER -

Multiplexed Detection

Abstract

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Keywords

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