TY - JOUR
T1 - Multiplexed detection
T2 - fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns
AU - Pravadali-Cekic, Sercan
AU - Kocic, Danijela
AU - Conlan, Xavier
AU - Shalliker, R. Andrew
N1 - Publisher Copyright:
© 2015 Taylor & Francis Group, LLC.
PY - 2015/11/26
Y1 - 2015/11/26
N2 - Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH"¢ for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.
AB - Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH"¢ for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.
KW - chemistry, analytic
KW - high performance liquid chromatography
KW - multiplexing
KW - tobacco
UR - http://handle.uws.edu.au:8081/1959.7/uws:32990
UR - http://www.scopus.com/inward/record.url?scp=84948950347&partnerID=8YFLogxK
U2 - 10.1080/10826076.2015.1105256
DO - 10.1080/10826076.2015.1105256
M3 - Article
SN - 1082-6076
VL - 38
SP - 1753
EP - 1758
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 19
ER -