TY - JOUR
T1 - Narrow bore post column derivatisation assays via reaction flow chromatography
AU - Acquaviva, Agustín
AU - Jones, Andrew
AU - Dennis, Gary R.
AU - Castells, Cecilia
AU - Shalliker, R. Andrew
AU - Soliven, Arianne
PY - 2018
Y1 - 2018
N2 - This study highlights the capability to conduct a post column derivatisation (PCD) process with a narrow bore, low volume column. A reaction flow (RF) HPLC column (30 mm à 2.1 mm internal diameter (i.d.), 3 μm particle diameter) enabled the selective detection of phenols for both a simple standard mixture and a complex sample represented by green tea. The resolving power and sensitivity obtained were similar to an analytical scale (4.6 mm i.d.) RF HPLC column of the same format. However, the baseline noise experienced was higher when the assay was scaled down from the RF 4.6 mm i.d. to the RF 2.1 mm i.d. narrow bore column. The PCD mixing and reaction speed inside the RF column outlet (frit and head-fitting design) enabled the coupling of PCD protocols with “UHPLC-type” low volume columns and systems without significant loss in separation performance. In comparison to a conventional analytical scale PCD approach the scaled down narrow bore RF approach demonstrated 40 times greater sensitivity in terms of S/N. Scaled down PCD is vital for decreased consumption and cost without significant loss in separation performance.
AB - This study highlights the capability to conduct a post column derivatisation (PCD) process with a narrow bore, low volume column. A reaction flow (RF) HPLC column (30 mm à 2.1 mm internal diameter (i.d.), 3 μm particle diameter) enabled the selective detection of phenols for both a simple standard mixture and a complex sample represented by green tea. The resolving power and sensitivity obtained were similar to an analytical scale (4.6 mm i.d.) RF HPLC column of the same format. However, the baseline noise experienced was higher when the assay was scaled down from the RF 4.6 mm i.d. to the RF 2.1 mm i.d. narrow bore column. The PCD mixing and reaction speed inside the RF column outlet (frit and head-fitting design) enabled the coupling of PCD protocols with “UHPLC-type” low volume columns and systems without significant loss in separation performance. In comparison to a conventional analytical scale PCD approach the scaled down narrow bore RF approach demonstrated 40 times greater sensitivity in terms of S/N. Scaled down PCD is vital for decreased consumption and cost without significant loss in separation performance.
KW - chromatographic analysis
KW - phenols
UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:47848
U2 - 10.1016/j.microc.2018.06.032
DO - 10.1016/j.microc.2018.06.032
M3 - Article
VL - 142
SP - 181
EP - 186
JO - Microchemical Journal
JF - Microchemical Journal
ER -