Novel exopolysaccharide produced from fermented bamboo shoot-isolated Lactobacillus fermentum

Thi Bich Thuy Do; Thi Ai Luyen Tran; Thi Van Thi Tran; Trung Hieu Le; Vijay Jayasena; Thi Hong Chuong Nguyen; Chinh Chien Nguyen; Soo Young Kim; Quyet Van Le

doi:10.3390/polym12071531

Novel exopolysaccharide produced from fermented bamboo shoot-isolated Lactobacillus fermentum

Thi Bich Thuy Do, Thi Ai Luyen Tran, Thi Van Thi Tran, Trung Hieu Le, Vijay Jayasena, Thi Hong Chuong Nguyen, Chinh Chien Nguyen, Soo Young Kim, Quyet Van Le

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in theBCCM/LMGBacteria Collection, Ghent University, Belgiumby the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 10₄ Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]_n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.

Original language	English
Article number	1531
Pages (from-to)	1-16
Number of pages	16
Journal	Polymers
Volume	12
Issue number	7
DOIs	https://doi.org/10.3390/polym12071531
Publication status	Published - 1 Jul 2020

Bibliographical note

Publisher Copyright:
© 2020 by the authors.

Open Access - Access Right Statement

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

gas chromatography
lactic acid bacteria
mass spectrometry
microbial exopolysaccharides
nuclear magnetic resonance spectroscopy

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10.3390/polym12071531

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title = "Novel exopolysaccharide produced from fermented bamboo shoot-isolated Lactobacillus fermentum",

abstract = "This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in theBCCM/LMGBacteria Collection, Ghent University, Belgiumby the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 104 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.",

keywords = "gas chromatography, lactic acid bacteria, mass spectrometry, microbial exopolysaccharides, nuclear magnetic resonance spectroscopy",

author = "Do, {Thi Bich Thuy} and Tran, {Thi Ai Luyen} and Tran, {Thi Van Thi} and Le, {Trung Hieu} and Vijay Jayasena and Nguyen, {Thi Hong Chuong} and Nguyen, {Chinh Chien} and Kim, {Soo Young} and Le, {Quyet Van}",

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AU - Do, Thi Bich Thuy

AU - Tran, Thi Ai Luyen

AU - Tran, Thi Van Thi

AU - Le, Trung Hieu

AU - Jayasena, Vijay

AU - Nguyen, Thi Hong Chuong

AU - Nguyen, Chinh Chien

AU - Kim, Soo Young

AU - Le, Quyet Van

PY - 2020/7/1

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N2 - This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in theBCCM/LMGBacteria Collection, Ghent University, Belgiumby the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 104 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.

AB - This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in theBCCM/LMGBacteria Collection, Ghent University, Belgiumby the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 104 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.

KW - gas chromatography

KW - lactic acid bacteria

KW - mass spectrometry

KW - microbial exopolysaccharides

KW - nuclear magnetic resonance spectroscopy

UR - http://hdl.handle.net/1959.7/uws:56706

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DO - 10.3390/polym12071531

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M1 - 1531

ER -

Novel exopolysaccharide produced from fermented bamboo shoot-isolated Lactobacillus fermentum

Abstract

Bibliographical note

Open Access - Access Right Statement

Keywords

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