Nuclear translocation of NF-ϰB in intact human gut tissue upon stimulation with coffee and roasting products

Tanja Sauer, Martin Raithel, Jürgen Kressel, Sonja Muscat, Gerald Münch, Monika Pischetsrieder

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    In the healthy gut, NF-ϰB is a critical factor of the intestinal immune system, whereas inflammatory bowel diseases are associated with chronic activation of NF-ϰB. Previous studies indicated that coffee induces nuclear translocation of NF-ϰB in macrophages, an effect attributed to roasting products. In the present work, coffee extract or roasting products induced nuclear translocation of NF-ϰB in macrophages, Caco-2 cells, and primary human intestinal microvascular endothelial cells (up to fivefold, p ˂ 0.001). Since the effect clearly depended on the cell type, ex vivo experiments were performed with intact human gut tissue from biopsies. The uniformity of the specimens and tissue viability during ex vivo incubation for up to 2 h were verified. Roasting products led to a concentration dependent significant increase of nuclear translocation of NF-ϰB in human gut tissue (up to 2.85 fold increase, p = 0.0321), whereas coffee extract induced a trend towards higher nuclear NF-ϰB concentration. NF-ϰB activation in macrophages and Caco-2 cells by roasting products was significantly blocked by co-incubation with catalase (p = 0.011 and p = 0.024) indicating involvement of H2O2-signaling. Monitoring of extracellular H2O2 indicated that roasting products in coffee constantly generate H2O2 by spontaneous oxygen reduction, which is only partially detoxified by cellular antioxidative systems. Thus, it can be concluded that ex vivo stimulation of intact human gut tissue is a valuable model to study nutritional effects on complex tissue systems. Furthermore, the consumption of coffee and roasting products may be able to induce nuclear NF-ϰB translocation in the human gut.
    Original languageEnglish
    Pages (from-to)529-540
    Number of pages12
    JournalFood and Function
    Volume2
    Issue number9
    DOIs
    Publication statusPublished - 2011

    Keywords

    • coffee
    • human guts
    • immune system
    • inflammatory bowel diseases
    • mucosa oxygenation
    • nuclear translocation
    • tissue

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