TY - JOUR
T1 - Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology
AU - Vuong, Quan V.
AU - Nguyen, Van Tang
AU - Thanh, Dang Trung
AU - Bhuyan, Deep Jyoti
AU - Goldsmith, Chloe D.
AU - Sadeqzadeh, Elham
AU - Scarlett, Christopher J.
AU - Bowyer, Michael C.
PY - 2015
Y1 - 2015
N2 - Euphol identified in Euphorbia tirucalli (E. tirucalli) has been linked with various health benefits. This study aimed to optimize ultrasonic extraction conditions for euphol from E. tirucalli leaf. Different solvents were tested to determine the most effective solvent for extraction of euphol. Then, response surface methodology (RSM) was employed to optimize ultrasound-assisted extraction conditions including temperature, time and power for maximal extraction of euphol. Our results showed that ethyl acetate:ethanol (4:1,v/v) was the most effective solvent for the extraction of euphol. Ultrasonic temperature and time had a positive impact, whereas, ultrasonic power had a negative effect on the extraction efficiency of euphol. The optimum ultrasonic extraction conditions for euphol were identified as: solvent-to-fresh sample ratio of 100:32 mL/g; ultrasonic temperature of 60◦C; ultrasonic time of 75 min and ultrasonic power of60% (150 W). Under these optimum conditions, approximately 4.06 mg of euphol could be obtained from one gram of fresh E. tirucalli leaf. This extract also contained phenolic compounds (2.5 mg GAE/g FW)and possessed potent antioxidant capacity. These optimal conditions are applicable for a larger scale to extract and isolate euphol for potential utilization in the pharmaceutical industry.
AB - Euphol identified in Euphorbia tirucalli (E. tirucalli) has been linked with various health benefits. This study aimed to optimize ultrasonic extraction conditions for euphol from E. tirucalli leaf. Different solvents were tested to determine the most effective solvent for extraction of euphol. Then, response surface methodology (RSM) was employed to optimize ultrasound-assisted extraction conditions including temperature, time and power for maximal extraction of euphol. Our results showed that ethyl acetate:ethanol (4:1,v/v) was the most effective solvent for the extraction of euphol. Ultrasonic temperature and time had a positive impact, whereas, ultrasonic power had a negative effect on the extraction efficiency of euphol. The optimum ultrasonic extraction conditions for euphol were identified as: solvent-to-fresh sample ratio of 100:32 mL/g; ultrasonic temperature of 60◦C; ultrasonic time of 75 min and ultrasonic power of60% (150 W). Under these optimum conditions, approximately 4.06 mg of euphol could be obtained from one gram of fresh E. tirucalli leaf. This extract also contained phenolic compounds (2.5 mg GAE/g FW)and possessed potent antioxidant capacity. These optimal conditions are applicable for a larger scale to extract and isolate euphol for potential utilization in the pharmaceutical industry.
KW - Euphorbiaceae
KW - Euphol
KW - Ultrasonic-assisted extraction
KW - Euphorbia tirucalli
KW - Response surface methodology
KW - Optimization
UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:48346
UR - http://www.scopus.com/inward/record.url?scp=84922318977&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2014.09.057
DO - 10.1016/j.indcrop.2014.09.057
M3 - Article
SN - 0926-6690
VL - 63
SP - 197
EP - 202
JO - Industrial Crops and Products
JF - Industrial Crops and Products
ER -